• Immunofluorescence analysis of 293T-SARS-CoV-2 NSP13 (His-tag) and 293T cells using SARS-CoV-2 NSP13 Rabbit polyclonal antibody (STJ11103267) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of normal 293T cells and 293T transfected with NSP13 Protein, using SARS-CoV-2 NSP13 antibody (STJ11103267) at 1:5000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-SARS-CoV-2-NSP13 antibody (1-601) (STJ11103267)

SKU:
STJ11103267

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Host: Rabbit
Applications: WB/IF
Reactivity: SARS-CoV-2
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SARS-CoV-2-NSP13 (1-601) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:2000-1:6000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen Region: 1-601
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-601 of coronavirus NSP13 (YP_009725308.1).
Immunogen Sequence: AVGACVLCNSQTSLRCGACI RRPFLCCKCCYDHVISTSHK LVLSVNPYVCNAPGCDVTDV TQLYLGGMSYYCKSHKPPIS FPLCANGQVFGLYKNTCVGS DNVTDFNAIATCDWTNAGDY ILANTCTERLKLFAAETLKA TEETFKLSYGIATVREVLSD RELHLSWEVGKPRPPLNRNY VFTGYRVTKNSKVQIGEYTF EKGDYGDAVVYRGTTTYKLN VGDYFVLTSHTVMPLSAPT
Background The protein encoded by this gene is of unknown function. However, it is orthologous to the mouse Trmt2a gene and contains an RNA methyltransferase domain. Expression of this gene varies during the cell cycle, with aberrant expression being a possible biomarker in certain breast cancers. Several transcript variants encoding two different isoforms have been found for this gene.

Information sourced from Uniprot.org

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