Anti-SAFB antibody [2E8-E2-G6] (STJ99122)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG1
ImmunogenPurified recombinant human SAFB1 protein fragments expressed in E.coli.
General Information
| Short Description | Mouse monoclonal anti-Scaffold attachment factor B1 for use in WB and ICC in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ICC |
| Host | Mouse |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | 2E8-E2-G6 |
| Isotype | IgG1 |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:1000ICC 1:200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | SAFB |
| Gene ID | 6294 |
| Uniprot ID | SAFB1_HUMAN |
| Immunogen | Purified recombinant human SAFB1 protein fragments expressed in E.coli. |
| Specificity | This antibody detects endogenous levels of SAFB1 and does not cross-react with related prote |
Additional Info
| Post Translational Modifications | Sumoylated by PIAS1 with SUMO1 and SUMO2/3, desumoylated by SENP1. Sumoylation is required for transcriptional repressor activity. |
| Function | Binds to scaffold/matrix attachment region (S/MAR) DNA and forms a molecular assembly point to allow the formation of a 'transcriptosomal' complex (consisting of SR proteins and RNA polymerase II) coupling transcription and RNA processing. Functions as an estrogen receptor corepressor and can also bind to the HSP27 promoter and decrease its transcription. Thereby acts as a negative regulator of cell proliferation. When associated with RBMX, binds to and stimulates transcription from the SREBF1 promoter. |
| Protein Name | Scaffold Attachment Factor B1Saf-BSaf-B1Hsp27 Estrogen Response Element-Tata Box-Binding ProteinHsp27 Ere-Tata-Binding Protein |
| Database Links | Reactome: R-HSA-3899300 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Scaffold Attachment Factor B1 antibodyAnti-Saf-B antibodyAnti-Saf-B1 antibodyAnti-Hsp27 Estrogen Response Element-Tata Box-Binding Protein antibodyAnti-Hsp27 Ere-Tata-Binding Protein antibodyAnti-SAFB antibodyAnti-HAP antibodyAnti-HET antibodyAnti-SAFB1 antibody |
Information sourced from Uniprot.org