Anti-SAE1 antibody (221-270 aa) (STJ95572)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human AOS1 at the amino acid range 221-270
General Information
| Short Description | Rabbit polyclonal anti-SUMO-activating enzyme subunit 1 (221-270 aa) for use in WB, IHC, IF and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC 1:100-1:300IF 1:200-1:1000ELISA 1:20000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | SAE1 |
| Gene ID | 10055 |
| Uniprot ID | SAE1_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human AOS1 at the amino acid range 221-270 |
| Immunogen Region | 221-270 aa |
| Specificity | SAE1 Polyclonal Antibody detects endogenous levels of SAE1 protein. |
Additional Info
| Function | The heterodimer acts as an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2. |
| Protein Name | Sumo-Activating Enzyme Subunit 1Ubiquitin-Like 1-Activating Enzyme E1a Cleaved Into - Sumo-Activating Enzyme Subunit 1 - N-Terminally Processed |
| Database Links | Reactome: R-HSA-3065676Reactome: R-HSA-3065678 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Sumo-Activating Enzyme Subunit 1 antibodyAnti-Ubiquitin-Like 1-Activating Enzyme E1a Cleaved Into - Sumo-Activating Enzyme Subunit 1 - N-Terminally Processed antibodyAnti-SAE1 antibodyAnti-AOS1 antibodyAnti-SUA1 antibodyAnti-UBLE1A antibody |
Information sourced from Uniprot.org