• Fluorescence multiplex immunohistochemical analysis of mouse brain tissue (formalin-fixed paraffin-embedded section). The immunostaining was performed by Sextuple-Fluorescence kit. GFAP mouse monoclonal antibody (STJA0006242 green), S100 mouse monoclonal antibody (STJ197266 cyan), Neurofilament mouse monoclonal antibody (purple), Iba 1 mouse monoclonal antibody (red), CD8 a mouse monoclonal antibody (yellow), The section was incubated in 5 rounds of staining; sequentially for Anti-antibodies; each using a separate fluorescent tyramide signal amplification system. EDTA based antigen retrieval (pH 9.0, 20 minutes) was used in between rounds of tyramide signal amplification to remove the antibody from the previous round, to avoid any cross-reactivity. DAPI (dark blue) was used as a nuclear counter stain. Microscopy and pseudocoloring of individual dyes was performed using a Slideviewer Imaging System (Excilone).
  • Human appendix tissue was stained with Anti-S100 (ABT094) Antibody
  • Human malignant melanoma tissue was stained with Anti-S100 (ABT094) Antibody
  • Human malignant melanoma tissue was stained with Anti-S100 (ABT094) Antibody
  • Human malignant melanoma tissue was stained with Anti-S100 (ABT094) Antibody
  • Human tonsil tissue was stained with Anti-S100 (ABT094) Antibody

Anti-S100B antibody (2-92 aa) [ABT094] (STJ197266)

SKU:
STJ197266

£168.00 - £226.00
Current Stock:
Host: Mouse
Applications: IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Protein S100-B (2-92 aa) is suitable for use in Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: ABT094
Conjugation: Unconjugated
Isotype: IgG2ak
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Dilution Range: IHC-P 1:100-500
IF 1:100-500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: S100B
Gene ID: 6285
Uniprot ID: S100B_HUMAN
Immunogen Region: 2-92 aa
Specificity: The antibody can specifically recognize human S100B protein, and has a weak reaction with S100A1, but no cross reaction with S100A4 or S100A6.
Immunogen: Synthesized peptide derived from the human S100 at the amino acid range 2-92
Function Small zinc- and- and calcium-binding protein that is highly expressed in astrocytes and constitutes one of the most abundant soluble proteins in brain. Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Acts as a neurotrophic factor that promotes astrocytosis and axonal proliferation. Involved in innervation of thermogenic adipose tissue by acting as an adipocyte-derived neurotrophic factor that promotes sympathetic innervation of adipose tissue. Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. Could assist ATAD3A cytoplasmic processing, preventing aggregation and favoring mitochondrial localization. May mediate calcium-dependent regulation on many physiological processes by interacting with other proteins, such as TPR-containing proteins, and modulating their activity.
Protein Name Protein S100-B
S-100 Protein Beta Chain
S-100 Protein Subunit Beta
S100 Calcium-Binding Protein B
Database Links Reactome: R-HSA-1251985
Reactome: R-HSA-445989
Reactome: R-HSA-879415
Reactome: R-HSA-933542
Cellular Localisation Cytoplasm
Nucleus
Secreted
Secretion Into The Medium Is Promoted By Interaction With Isoform Clstn3beta Of Clstn3
Alternative Antibody Names Anti-Protein S100-B antibody
Anti-S-100 Protein Beta Chain antibody
Anti-S-100 Protein Subunit Beta antibody
Anti-S100 Calcium-Binding Protein B antibody
Anti-S100B antibody

Information sourced from Uniprot.org

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