Western blot analysis of extracts of various cell lines, using S100A10 antibody (STJ25429) at 1:400 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-S100A10 antibody (1-97) (STJ25429)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ25429
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General Information

Short DescriptionRabbit polyclonal S100A10 (1-97) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse, rat and monkey samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/IHC-P/IF/ICC/ELISA
HostRabbit
ReactivityHuman/Mouse/Rat/Monkey
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:500-1:1000
IHC-P:1:50-1:200
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolS100A10
Gene ID6281
Uniprot IDS10AA_HUMAN
Immunogen Region1-97
Immunogen SequenceMPSQMEHAMETMMFTFHKFA GDKGYLTKEDLRVLMEKEFP GFLENQKDPLAVDKIMKDLD QCRDGKVGFQSFFSLIAGLT IACNDYFVVHMKQKGKK
SpecificityRecombinant fusion protein containing a sequence corresponding to amino acids 1-97 of human S100A10 (NP_002957.1).

Additional Info

Function Because S100A10 induces the dimerization of ANXA2/p36, it may function as a regulator of protein phosphorylation in that the ANXA2 monomer is the preferred target (in vitro) of tyrosine-specific kinase.
Protein Name Protein S100-A10
Calpactin I Light Chain
Calpactin-1 Light Chain
Cellular Ligand Of Annexin Ii
S100 Calcium-Binding Protein A10
P10 Protein
P11
Database Links Reactome: R-HSA-75205
Cellular Localisation
Alternative Antibody Names Anti-Protein S100-A10 antibody
Anti-Calpactin I Light Chain antibody
Anti-Calpactin-1 Light Chain antibody
Anti-Cellular Ligand Of Annexin Ii antibody
Anti-S100 Calcium-Binding Protein A10 antibody
Anti-P10 Protein antibody
Anti-P11 antibody
Anti-S100A10 antibody
Anti-ANX2LG antibody
Anti-CAL1L antibody
Anti-CLP11 antibody

Information sourced from Uniprot.org

Citations

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