• Western blot analysis of extracts of various cell lines, using S100A10 antibody (STJ25429) at 1:400 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of extracts of various cell lines, using S100A10 antibody (STJ25429) at 1:400 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Immunohistochemistry analysis of paraffin-embedded human esophagus using S100A10 rabbit polyclonal antibody (STJ25429) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human lung using S100A10 rabbit polyclonal antibody (STJ25429) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human placenta using S100A10 rabbit polyclonal antibody (STJ25429) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of HT-29 cells using S100A10 rabbit polyclonal antibody (STJ25429) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using S100A10 rabbit polyclonal antibody (STJ25429) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-S100A10 antibody (1-97) (STJ25429)

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STJ25429

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat/Monkey
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-S100A10 (1-97) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: S100A10
Gene ID: 6281
Uniprot ID: S10AA_HUMAN
Immunogen Region: 1-97
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-97 of human S100A10 (NP_002957.1).
Immunogen Sequence: MPSQMEHAMETMMFTFHKFA GDKGYLTKEDLRVLMEKEFP GFLENQKDPLAVDKIMKDLD QCRDGKVGFQSFFSLIAGLT IACNDYFVVHMKQKGKK
Function Because S100A10 induces the dimerization of ANXA2/p36, it may function as a regulator of protein phosphorylation in that the ANXA2 monomer is the preferred target (in vitro) of tyrosine-specific kinase.
Protein Name Protein S100-A10
Calpactin I Light Chain
Calpactin-1 Light Chain
Cellular Ligand Of Annexin Ii
S100 Calcium-Binding Protein A10
P10 Protein
P11
Database Links Reactome: R-HSA-75205
Alternative Antibody Names Anti-Protein S100-A10 antibody
Anti-Calpactin I Light Chain antibody
Anti-Calpactin-1 Light Chain antibody
Anti-Cellular Ligand Of Annexin Ii antibody
Anti-S100 Calcium-Binding Protein A10 antibody
Anti-P10 Protein antibody
Anti-P11 antibody
Anti-S100A10 antibody
Anti-ANX2LG antibody
Anti-CAL1L antibody
Anti-CLP11 antibody

Information sourced from Uniprot.org

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