| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: | Monoclonal |
| Clone ID: | SRM |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.09% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:1000-1:6000 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Gene Symbol: | RUNX1 |
| Gene ID: | 861 |
| Uniprot ID: | RUNX1_HUMAN |
| Immunogen Region: | 101-200 aa |
| Specificity: | A synthetic peptide corresponding to a sequence within amino acids 101-200 of human RUNX1 (NP_001001890.1). |
| Immunogen Sequence: | GTLVTVMAGNDENYSAELRN ATAAMKNQVARFNDLRFVGR SGRGKSFTLTITVFTNPPQV ATYHRAIKITVDGPREPRRH RQKLDDQTKPGSLSFSERLS E |
| Tissue Specificity | Expressed in all tissues examined except brain and heart. Highest levels in thymus, bone marrow and peripheral blood. |
| Post Translational Modifications | Phosphorylated in its C-terminus upon IL-6 treatment. Phosphorylation enhances interaction with KAT6A. Methylated. Phosphorylated in Ser-249 Thr-273 and Ser-276 by HIPK2 when associated with CBFB and DNA. This phosphorylation promotes subsequent EP300 phosphorylation. |
| Function | Forms the heterodimeric complex core-binding factor (CBF) with CBFB. RUNX members modulate the transcription of their target genes through recognizing the core consensus binding sequence 5'-TGTGGT-3', or very rarely, 5'-TGCGGT-3', within their regulatory regions via their runt domain, while CBFB is a non-DNA-binding regulatory subunit that allosterically enhances the sequence-specific DNA-binding capacity of RUNX. The heterodimers bind to the core site of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM-CSF promoters (Probable). Essential for the development of normal hematopoiesis. Acts synergistically with ELF4 to transactivate the IL-3 promoter and with ELF2 to transactivate the BLK promoter. Inhibits KAT6B-dependent transcriptional activation. Involved in lineage commitment of immature T cell precursors. CBF complexes repress ZBTB7B transcription factor during cytotoxic (CD8+) T cell development. They bind to RUNX-binding sequence within the ZBTB7B locus acting as transcriptional silencer and allowing for cytotoxic T cell differentiation. CBF complexes binding to the transcriptional silencer is essential for recruitment of nuclear protein complexes that catalyze epigenetic modifications to establish epigenetic ZBTB7B silencing. Controls the anergy and suppressive function of regulatory T-cells (Treg) by associating with FOXP3. Activates the expression of IL2 and IFNG and down-regulates the expression of TNFRSF18, IL2RA and CTLA4, in conventional T-cells. Positively regulates the expression of RORC in T-helper 17 cells. Isoform AML-1G shows higher binding activities for target genes and binds TCR-beta-E2 and RAG-1 target site with threefold higher affinity than other isoforms. It is less effective in the context of neutrophil terminal differentiation. Isoform AML-1L interferes with the transactivation activity of RUNX1. |
| Protein Name | Runt-Related Transcription Factor 1 Acute Myeloid Leukemia 1 Protein Core-Binding Factor Subunit Alpha-2 Cbf-Alpha-2 Oncogene Aml-1 Polyomavirus Enhancer-Binding Protein 2 Alpha B Subunit Pea2-Alpha B Pebp2-Alpha B Sl3-3 Enhancer Factor 1 Alpha B Subunit Sl3/Akv Core-Binding Factor Alpha B Subunit |
| Database Links | Reactome: R-HSA-1912408 Reactome: R-HSA-549127 Reactome: R-HSA-8877330 Reactome: R-HSA-8931987 Reactome: R-HSA-8934593 Reactome: R-HSA-8935964 Reactome: R-HSA-8936459 Reactome: R-HSA-8939236 Reactome: R-HSA-8939242 Reactome: R-HSA-8939243 Reactome: R-HSA-8939245 Reactome: R-HSA-8939246 Reactome: R-HSA-8939247 Reactome: R-HSA-8939256 Reactome: R-HSA-8941333 Reactome: R-HSA-8951936 Reactome: R-HSA-9018519 Reactome: R-HSA-9616222 Reactome: R-HSA-9692916 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Runt-Related Transcription Factor 1 antibody Anti-Acute Myeloid Leukemia 1 Protein antibody Anti-Core-Binding Factor Subunit Alpha-2 antibody Anti-Cbf-Alpha-2 antibody Anti-Oncogene Aml-1 antibody Anti-Polyomavirus Enhancer-Binding Protein 2 Alpha B Subunit antibody Anti-Pea2-Alpha B antibody Anti-Pebp2-Alpha B antibody Anti-Sl3-3 Enhancer Factor 1 Alpha B Subunit antibody Anti-Sl3/Akv Core-Binding Factor Alpha B Subunit antibody Anti-RUNX1 antibody Anti-AML1 antibody Anti-CBFA2 antibody |
Information sourced from Uniprot.org