Anti-RUBCN antibody (1-200) (STJ115135)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal Rubicon (1-200) antibody for use in WB and ELISA in mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.09% Sodium Azide, 50% Glycerol, pH7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | RUBCN |
| Gene ID | 9711 |
| Uniprot ID | RUBIC_HUMAN |
| Immunogen Region | 1-200 |
| Immunogen Sequence | MRPEGAGMELGGGEERLPEE SRREHWQLLGNLKTTVEGLV STNSPNVWSKYGGLERLCRD MQSILYHGLIRDQACRRQTD YWQFVKDIRWLSPHSALHVE KFISVHENDQSSADGASERA VAELWLQHSLQYHCLSAQLR PLLGDRQYIRKFYTDAAFLL SDAHVTAMLQCLEAVEQNNP RLLAQIDASMFARKHESPLL |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human Rubicon (NP_055502.1). |
Additional Info
| Function | Inhibits PIK3C3 activity.under basal conditions negatively regulates PI3K complex II (PI3KC3-C2) function in autophagy. Negatively regulates endosome maturation and degradative endocytic trafficking and impairs autophagosome maturation process. Can sequester UVRAG from association with a class C Vps complex (possibly the HOPS complex) and negatively regulates Rab7 activation. Involved in regulation of pathogen-specific host defense of activated macrophages. Following bacterial infection promotes NADH oxidase activity by association with CYBA thereby affecting TLR2 signaling and probably other TLR-NOX pathways. Stabilizes the CYBA:CYBB NADPH oxidase heterodimer, increases its association with TLR2 and its phagosome trafficking to induce antimicrobial burst of ROS and production of inflammatory cytokines. Following fungal or viral infection (implicating CLEC7A (dectin-1)-mediated myeloid cell activation or RIGI-dependent sensing of RNA viruses) negatively regulates pro-inflammatory cytokine production by association with CARD9 and sequestering it from signaling complexes. |
| Protein Name | Run Domain Beclin-1-Interacting And Cysteine-Rich Domain-Containing ProteinRubiconBeclin-1 Associated Run Domain Containing ProteinBaron |
| Database Links | |
| Cellular Localisation | Late EndosomeLysosomeEarly EndosomePredominantly Located In Late Endosomes/LysosomesOnly Partially Detected In Early Endosome And Not At All In The Golgi Apparatus |
| Alternative Antibody Names | Anti-Run Domain Beclin-1-Interacting And Cysteine-Rich Domain-Containing Protein antibodyAnti-Rubicon antibodyAnti-Beclin-1 Associated Run Domain Containing Protein antibodyAnti-Baron antibodyAnti-RUBCN antibodyAnti-KIAA0226 antibody |
Information sourced from Uniprot.org