• Western blot analysis of lysates from Raw264.7 cells, treated with UV 5', using MSK2 Antibody. The lane on the right is blocked with the synthesized peptide.

Anti-RPS6KA4 antibody (531-580) (STJ94268)

SKU:
STJ94268

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Ribosomal protein S6 kinase alpha-4 (531-580) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:5000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RPS6KA4
Gene ID: 8986
Uniprot ID: KS6A4_HUMAN
Immunogen Region: 531-580
Specificity: MSK2 Polyclonal Antibody detects endogenous levels of MSK2 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from human MSK2. AA range:531-580
Post Translational Modifications Ser-343 and Thr-568 phosphorylation is required for kinase activity. Ser-343 and Ser-196 are autophosphorylated by the C-terminal kinase domain, and their phosphorylation is essential for the catalytic activity of the N-terminal kinase domain. Phosphorylated at Ser-343, Thr-568 and Thr-687 by MAPK1/ERK2, MAPK3/ERK1 and MAPK14/p38-alpha. Autophosphorylated at Ser-737 and Ser-745 by the N-terminal kinase domain.
Function Serine/threonine-protein kinase that is required for the mitogen or stress-induced phosphorylation of the transcription factors CREB1 and ATF1 and for the regulation of the transcription factor RELA, and that contributes to gene activation by histone phosphorylation and functions in the regulation of inflammatory genes. Phosphorylates CREB1 and ATF1 in response to mitogenic or stress stimuli such as UV-C irradiation, epidermal growth factor (EGF) and anisomycin. Plays an essential role in the control of RELA transcriptional activity in response to TNF. Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and EGF, which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN. May also phosphorylate 'Ser-28' of histone H3. Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 1 (HMGN1/HMG14). In lipopolysaccharide-stimulated primary macrophages, acts downstream of the Toll-like receptor TLR4 to limit the production of pro-inflammatory cytokines. Functions probably by inducing transcription of the MAP kinase phosphatase DUSP1 and the anti-inflammatory cytokine interleukin 10 (IL10), via CREB1 and ATF1 transcription factors.
Protein Name Ribosomal Protein S6 Kinase Alpha-4
S6k-Alpha-4
90 Kda Ribosomal Protein S6 Kinase 4
Nuclear Mitogen- And Stress-Activated Protein Kinase 2
Ribosomal Protein Kinase B
Rskb
Database Links Reactome: R-HSA-437239
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Ribosomal Protein S6 Kinase Alpha-4 antibody
Anti-S6k-Alpha-4 antibody
Anti-90 Kda Ribosomal Protein S6 Kinase 4 antibody
Anti-Nuclear Mitogen- And Stress-Activated Protein Kinase 2 antibody
Anti-Ribosomal Protein Kinase B antibody
Anti-Rskb antibody
Anti-RPS6KA4 antibody
Anti-MSK2 antibody

Information sourced from Uniprot.org

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