• Western blot analysis of extracts of various cell lines, using RPS18 antibody (STJ113287) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer using RPS18 antibody (STJ113287) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse kidney using RPS18 antibody (STJ113287) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded Rat brain using RPS18 antibody (STJ113287) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of HepG2 cells using RPS18 rabbit polyclonal antibody (STJ113287) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using RPS18 rabbit polyclonal antibody (STJ113287) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of PC-12 cells using RPS18 rabbit polyclonal antibody (STJ113287) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of THP-1 cells using RPS18 rabbit polyclonal antibody (STJ113287) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Anti-RPS18 antibody (1-152) (STJ113287)

SKU:
STJ113287

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RPS18 (1-152) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RPS18
Gene ID: 6222
Uniprot ID: RS18_HUMAN
Immunogen Region: 1-152
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-152 of human RPS18 (NP_072045.1).
Immunogen Sequence: MSLVIPEKFQHILRVLNTNI DGRRKIAFAITAIKGVGRRY AHVVLRKADIDLTKRAGELT EDEVERVITIMQNPRQYKIP DWFLNRQKDVKDGKYSQVLA NGLDNKLREDLERLKKIRAH RGLRHFWGLRVRGQHTKTTG RRGRTVGVSKKK
Function Component of the small ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell.
Protein Name Small Ribosomal Subunit Protein Us13
40s Ribosomal Protein S18
Ke-3
Ke3
Database Links Reactome: R-HSA-156827
Reactome: R-HSA-156902
Reactome: R-HSA-1799339
Reactome: R-HSA-192823
Reactome: R-HSA-2408557
Reactome: R-HSA-6791226
Reactome: R-HSA-72649
Reactome: R-HSA-72689
Reactome: R-HSA-72695
Reactome: R-HSA-72702
Reactome: R-HSA-72706
Reactome: R-HSA-72764
Reactome: R-HSA-9010553
Reactome: R-HSA-9633012
Reactome: R-HSA-9735869
Reactome: R-HSA-9754678
Reactome: R-HSA-975956
Reactome: R-HSA-975957
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Small Ribosomal Subunit Protein Us13 antibody
Anti-40s Ribosomal Protein S18 antibody
Anti-Ke-3 antibody
Anti-Ke3 antibody
Anti-RPS18 antibody
Anti-D6S218E antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance