• Immunofluorescence analysis of MCF7 cells using RPS14 antibody (STJ28810). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded mouse spinal cord using RPS14 Rabbit polyclonal antibody (STJ28810) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat pancreas using RPS14 Rabbit polyclonal antibody (STJ28810) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using RPS14 antibody (STJ28810) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-RPS14 antibody (1-151) (STJ28810)

SKU:
STJ28810

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RPS14 (1-151) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RPS14
Gene ID: 6208
Uniprot ID: RS14_HUMAN
Immunogen Region: 1-151
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-151 of human RPS14 (NP_005608.1).
Immunogen Sequence: MAPRKGKEKKEEQVISLGPQ VAEGENVFGVCHIFASFNDT FVHVTDLSGKETICRVTGGM KVKADRDESSPYAAMLAAQD VAQRCKELGITALHIKLRAT GGNRTKTPGPGAQSALRALA RSGMKIGRIEDVTPIPSDST RRKGGRRGRRL
Function Component of the small ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome.
Protein Name Small Ribosomal Subunit Protein Us11
40s Ribosomal Protein S14
Database Links Reactome: R-HSA-156827
Reactome: R-HSA-156902
Reactome: R-HSA-1799339
Reactome: R-HSA-192823
Reactome: R-HSA-2408557
Reactome: R-HSA-6790901
Reactome: R-HSA-6791226
Reactome: R-HSA-72649
Reactome: R-HSA-72689
Reactome: R-HSA-72695
Reactome: R-HSA-72702
Reactome: R-HSA-72706
Reactome: R-HSA-72764
Reactome: R-HSA-9010553
Reactome: R-HSA-9633012
Reactome: R-HSA-9735869
Reactome: R-HSA-9754678
Reactome: R-HSA-975956
Reactome: R-HSA-975957
Cellular Localisation Cytoplasm
Nucleus
Nucleolus
Alternative Antibody Names Anti-Small Ribosomal Subunit Protein Us11 antibody
Anti-40s Ribosomal Protein S14 antibody
Anti-RPS14 antibody
Anti-PRO2640 antibody

Information sourced from Uniprot.org

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