• Confocal immunofluorescence analysis of HeLa cells using RPS12 Polyclonal Antibody (STJ28163) at dilution of 1:400. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of MCF-7 cells using RPS12 antibody (STJ28163). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using RPS12 antibody (STJ28163) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

Anti-RPS12 antibody (1-132) (STJ28163)

SKU:
STJ28163

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RPS12 (1-132) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:10-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RPS12
Gene ID: 6206
Uniprot ID: RS12_HUMAN
Immunogen Region: 1-132
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-132 of human RPS12 (NP_001007.2).
Immunogen Sequence: MAEEGIAAGGVMDVNTALQE VLKTALIHDGLARGIREAAK ALDKRQAHLCVLASNCDEPM YVKLVEALCAEHQINLIKVD DNKKLGEWVGLCKIDREGKP RKVVGCSCVVVKDYGKESQA KDVIEEYFKCKK
Function Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome. Subunit of the 40S ribosomal complex.
Protein Name Small Ribosomal Subunit Protein Es12
40s Ribosomal Protein S12
Database Links Reactome: R-HSA-156827
Reactome: R-HSA-156902
Reactome: R-HSA-1799339
Reactome: R-HSA-192823
Reactome: R-HSA-2408557
Reactome: R-HSA-6791226
Reactome: R-HSA-72649
Reactome: R-HSA-72689
Reactome: R-HSA-72695
Reactome: R-HSA-72702
Reactome: R-HSA-72706
Reactome: R-HSA-72764
Reactome: R-HSA-9010553
Reactome: R-HSA-9633012
Reactome: R-HSA-9735869
Reactome: R-HSA-9754678
Reactome: R-HSA-975956
Reactome: R-HSA-975957
Cellular Localisation Nucleus
Nucleolus
Alternative Antibody Names Anti-Small Ribosomal Subunit Protein Es12 antibody
Anti-40s Ribosomal Protein S12 antibody
Anti-RPS12 antibody

Information sourced from Uniprot.org

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