| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-RPN1 (20-280) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
RPN1 |
| Gene ID: |
6184 |
| Uniprot ID: |
RPN1_HUMAN |
| Immunogen Region: |
20-280 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 20-280 of human RPN1 (NP_002941.1). |
| Immunogen Sequence: |
APGSASSEAPPLINEDVKRT VDLSSHLAKVTAEVVLAHLG GGSTSRATSFLLALEPELEA RLAHLGVQVKGEDEEENNLE VRETKIKGKSGRFFTVKLPV ALDPGAKISVIVETVYTHVL HPYPTQITQSEKQFVVFEGN HYFYSPYPTKTQTMRVKLAS RNVESYTKLGNPTRSEDLLD YGPFRDVPAYSQDTFKVHYE NNSPFLTITSMTRVIEVSHW GNIAVEENVDLKHTGAVLK |
| Tissue Specificity | Expressed in all tissues tested. |
| Post Translational Modifications | Ufmylated by UFL1 in response to endoplasmic reticulum stress, promoting reticulophagy of endoplasmic reticulum sheets. |
| Function | Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity. |
| Protein Name | Dolichyl-Diphosphooligosaccharide--Protein Glycosyltransferase Subunit 1Dolichyl-Diphosphooligosaccharide--Protein Glycosyltransferase 67 Kda SubunitRibophorin IRpn-IRibophorin-1 |
| Database Links | Reactome: R-HSA-1799339Reactome: R-HSA-446203Reactome: R-HSA-9694548 |
| Cellular Localisation | Endoplasmic ReticulumEndoplasmic Reticulum MembraneSingle-Pass Type I Membrane ProteinMelanosomeIdentified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv |
| Alternative Antibody Names | Anti-Dolichyl-Diphosphooligosaccharide--Protein Glycosyltransferase Subunit 1 antibodyAnti-Dolichyl-Diphosphooligosaccharide--Protein Glycosyltransferase 67 Kda Subunit antibodyAnti-Ribophorin I antibodyAnti-Rpn-I antibodyAnti-Ribophorin-1 antibodyAnti-RPN1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
