• Confocal immunofluorescence analysis of HeLa cells using RPL8 antibody (STJ112082) at dilution of 1:200. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using RPL8 antibody (STJ112082) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded mouse heart using RPL8 Rabbit polyclonal antibody (STJ112082) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse brain using RPL8 Rabbit polyclonal antibody (STJ112082) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using RPL8 Rabbit polyclonal antibody (STJ112082) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using RPL8 antibody (STJ112082) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-RPL8 antibody (1-257) (STJ112082)

SKU:
STJ112082

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RPL8 (1-257) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:4000
IHC-P 1:50-1:200
IF/ICC 1:100-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RPL8
Gene ID: 6132
Uniprot ID: RL8_HUMAN
Immunogen Region: 1-257
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-257 of human RPL8 (NP_000964.1).
Immunogen Sequence: MGRVIRGQRKGAGSVFRAHV KHRKGAARLRAVDFAERHGY IKGIVKDIIHDPGRGAPLAK VVFRDPYRFKKRTELFIAAE GIHTGQFVYCGKKAQLNIGN VLPVGTMPEGTIVCCLEEKP GDRGKLARASGNYATVISHN PETKKTRVKLPSGSKKVISS ANRAVVGVVAGGGRIDKPIL KAGRAYHKYKAKRNCWPRVR GVAMNPVEHPFGGGNHQHIG KPSTIRRDAPAGRKVGLIA
Post Translational Modifications Hydroxylated on His-216 by RIOX1. The modification is impaired by hypoxia.
Function Component of the large ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell.
Protein Name Large Ribosomal Subunit Protein Ul2
60s Ribosomal Protein L8
Database Links Reactome: R-HSA-156827
Reactome: R-HSA-156902
Reactome: R-HSA-1799339
Reactome: R-HSA-192823
Reactome: R-HSA-2408557
Reactome: R-HSA-6791226
Reactome: R-HSA-72689
Reactome: R-HSA-72706
Reactome: R-HSA-72764
Reactome: R-HSA-9010553
Reactome: R-HSA-9633012
Reactome: R-HSA-975956
Reactome: R-HSA-975957
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Large Ribosomal Subunit Protein Ul2 antibody
Anti-60s Ribosomal Protein L8 antibody
Anti-RPL8 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance