Anti-RNF213 antibody (307-407 aa) (STJ196631)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from the human RN213 at the amino acid range 307-407
General Information
| Short Description | Rabbit polyclonal anti-E3 ubiquitin-protein ligase RNF213 (307-407 aa) for use in IHC and IF in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/IF |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | IHC-P 1:50-200IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | RNF213 |
| Gene ID | 57674 |
| Uniprot ID | RN213_HUMAN |
| Immunogen | Synthesized peptide derived from the human RN213 at the amino acid range 307-407 |
| Immunogen Region | 307-407 aa |
| Specificity | This antibody detects endogenous levels of RN213 at Human/Mouse |
Additional Info
| Post Translational Modifications | Autoubiquitinated. |
| Function | Atypical E3 ubiquitin ligase that can catalyze ubiquitination of both proteins and lipids, and which is involved in various processes, such as lipid metabolism, angiogenesis and cell-autonomous immunity. Acts as a key immune sensor by catalyzing ubiquitination of the lipid A moiety of bacterial lipopolysaccharide (LPS) via its RZ-type zinc-finger: restricts the proliferation of cytosolic bacteria, such as Salmonella, by generating the bacterial ubiquitin coat through the ubiquitination of LPS. Also acts indirectly by mediating the recruitment of the LUBAC complex, which conjugates linear polyubiquitin chains. Ubiquitination of LPS triggers cell-autonomous immunity, such as antibacterial autophagy, leading to degradation of the microbial invader. Involved in lipid metabolism by regulating fat storage and lipid droplet formation.act by inhibiting the lipolytic process. Also regulates lipotoxicity by inhibiting desaturation of fatty acids. Also acts as an E3 ubiquitin-protein ligase via its RING-type zinc finger: mediates 'Lys-63'-linked ubiquitination of target proteins. Involved in the non-canonical Wnt signaling pathway in vascular development: acts by mediating ubiquitination and degradation of FLNA and NFATC2 downstream of RSPO3, leading to inhibit the non-canonical Wnt signaling pathway and promoting vessel regression. Also has ATPase activity.ATPase activity is required for ubiquitination of LPS. |
| Protein Name | E3 Ubiquitin-Protein Ligase Rnf213Alk Lymphoma Oligomerization Partner On Chromosome 17E3 Ubiquitin-Lipopolysaccharide Ligase Rnf213MysterinRing Finger Protein 213 |
| Database Links | Reactome: R-HSA-9635465Reactome: R-HSA-9725370Reactome: R-HSA-983168 |
| Cellular Localisation | CytoplasmCytosolLipid Droplet |
| Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Rnf213 antibodyAnti-Alk Lymphoma Oligomerization Partner On Chromosome 17 antibodyAnti-E3 Ubiquitin-Lipopolysaccharide Ligase Rnf213 antibodyAnti-Mysterin antibodyAnti-Ring Finger Protein 213 antibodyAnti-RNF213 antibodyAnti-ALO17 antibodyAnti-C17orf27 antibodyAnti-KIAA1554 antibodyAnti-KIAA1618 antibodyAnti-MYSTR antibody |
Information sourced from Uniprot.org