• Immunohistochemistry analysis of paraffin-embedded mouse liver using RNF126 Rabbit polyclonal antibody (STJ11105381) at dilution of 1:300 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human liver using RNF126 Rabbit polyclonal antibody (STJ11105381) at dilution of 1:300 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates, using RNF126 Rabbit polyclonal antibody (STJ11105381) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-RNF126 antibody (120-200) (STJ11105381)

SKU:
STJ11105381

£112.50 - £524.50
Current Stock:
Host: Rabbit
Applications: WB/IHC-P/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:1000-1:5000
IHC-P:1:100-1:500
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RNF126
Gene ID: 55658
Uniprot ID: RN126_HUMAN
Immunogen Region: 120-200
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 120-200 of human RNF126 (NP_919442.1).
Immunogen Sequence: HRYGARQPRARLTTRRATGR HEGVPTLEGIIQQLVNGIIT PATIPSLGPWGVLHSNPMDY AWGANGLDAIITQLLNQFEN T
Tissue Specificity Highly expressed in liver and testis.
Post Translational Modifications Ubiquitinated. May undergo autoubiquitination.
Function E3 ubiquitin-protein ligase that mediates ubiquitination oF target proteins. Depending on the associated E2 ligase, mediates 'Lys-27'-, 'Lys-29'-, 'Lys-48'- and/or 'Lys-63'-linked polyubiquitination of substrates. Part of a BAG6-dependent quality control process ensuring that proteins of the secretory pathway that are mislocalized to the cytosol are degraded by the proteasome. Probably acts by providing the ubiquitin ligase activity associated with the BAG6 complex and be responsible for ubiquitination of the hydrophobic mislocalized proteins and their targeting to the proteasome. May also play a role in the endosomal recycling of IGF2R, the cation-independent mannose-6-phosphate receptor. May play a role in the endosomal sorting and degradation of several membrane receptors including EGFR, FLT3, MET and CXCR4, by mediating their ubiquitination. By ubiquitinating CDKN1A/p21 and targeting it for degradation, may also promote cell proliferation. May monoubiquitinate AICDA. Acts as a regulator of DNA repair by mediating 'Lys-27'- and 'Lys-29'-linked polyubiquitination of MRE11, thereby promoting the exonuclease activity of MRE11.
Protein Name E3 Ubiquitin-Protein Ligase Rnf126
Ring Finger Protein 126
Database Links Reactome: R-HSA-983168
Cellular Localisation Cytoplasm
Nucleus
Alternative Antibody Names Anti-E3 Ubiquitin-Protein Ligase Rnf126 antibody
Anti-Ring Finger Protein 126 antibody
Anti-RNF126 antibody

Information sourced from Uniprot.org

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