Anti-RNF111 antibody (901-950 aa) (STJ91694)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human RNF111 at the amino acid range 901-950
General Information
| Short Description | Rabbit polyclonal anti-E3 ubiquitin-protein ligase Arkadia (901-950 aa) for use in WB and ELISA in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000ELISA 1:40000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | RNF111 |
| Gene ID | 54778 |
| Uniprot ID | RN111_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human RNF111 at the amino acid range 901-950 |
| Immunogen Region | 901-950 aa |
| Specificity | Arkadia Polyclonal Antibody detects endogenous levels of Arkadia protein. |
Additional Info
| Function | E3 ubiquitin-protein ligase. Required for mesoderm patterning during embryonic development. Acts as an enhancer of the transcriptional responses of the SMAD2/SMAD3 effectors, which are activated downstream of BMP. Acts by mediating ubiquitination and degradation of SMAD inhibitors such as SMAD7, inducing their proteasomal degradation and thereby enhancing the transcriptional activity of TGF-beta and BMP. In addition to enhance transcription of SMAD2/SMAD3 effectors, also regulates their turnover by mediating their ubiquitination and subsequent degradation, coupling their activation with degradation, thereby ensuring that only effectors 'in use' are degraded. Activates SMAD3/SMAD4-dependent transcription by triggering signal-induced degradation of SNON isoform of SKIL. Associates with UBE2D2 as an E2 enzyme. Specifically binds polysumoylated chains via SUMO interaction motifs (SIMs) and mediates ubiquitination of sumoylated substrates. Catalyzes 'Lys-63'-linked ubiquitination of sumoylated XPC in response to UV irradiation, promoting nucleotide excision repair. Mediates ubiquitination and degradation of sumoylated PML. The regulation of the BMP-SMAD signaling is however independent of sumoylation and is not dependent of SUMO interaction motifs (SIMs). |
| Protein Name | E3 Ubiquitin-Protein Ligase ArkadiaRing Finger Protein 111Hrnf111Ring-Type E3 Ubiquitin Transferase Arkadia |
| Database Links | Reactome: R-HSA-2173795Reactome: R-HSA-2173796Reactome: R-HSA-5696395Reactome: R-HSA-983168 |
| Cellular Localisation | NucleusCytoplasmPml BodyUpon Tgf-Beta TreatmentTranslocates From Nucleus To Cytosol |
| Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Arkadia antibodyAnti-Ring Finger Protein 111 antibodyAnti-Hrnf111 antibodyAnti-Ring-Type E3 Ubiquitin Transferase Arkadia antibodyAnti-RNF111 antibody |
Information sourced from Uniprot.org