| Short Description : | Goat polyclonal RNA polymerase sigma factor RpoD (330aa C-Term) antibody for use in WB in e.coli, pseudomonas and burkholderia samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB |
| Host: | Goat |
| Reactivity: | E.coli/Pseudomonas/Burkholderia |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | 3 mg/mL |
| Purification: | This antibody is epitope-affinity purified from goat antiserum. |
| Dilution Range: | WB 1:500-1:2000 |
| Formulation: | PBS, 20% Glycerol and 0.05% Sodium Azide. |
| Storage Instruction: | For continuous use, store at 2-8 C for one-two days. For extended storage, store in-20 C freezer. Working dilution samples should be discarded if not used within 12 hours. |
| Immunogen: | Recombinant peptide derived from within residues 330 aa to the C-terminus of rpoD (Escherichia) produced in E. coli. |
| Immunogen Region: | 330aa C-Term |
| Immunogen Sequence: | LHDVSEEVHRALQKLQQIEE HDVSEEVHRALQKLQQIEEE DVSEEVHRALQKLQQIEEET VSEEVHRALQKLQQIEEETG SEEVHRALQKLQQIEEETGL EEVHRALQKLQQIEEETGLT EVHRALQKLQQIEEETGLTI VHRALQKLQQIEEETGLTIE HRALQKLQQIEEETGLTIEQ RALQKLQQIEEETGLTIEQV ALQKLQQIEEETGLTIEQVK LQKLQQIEEETGLTIEQVK |
| Specificity: | Detects endogenous levels of rpoD by Western blot. |
| Background | Goat polyclonal antibody to RNA polymerase sigma factor rpoD. Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation. rpoD is the primary sigma factor during exponential growth. Preferentially transcribes genes associated with fast growth, such as ribosomal operons, other protein-synthesis related genes, rRNA-and tRNA-encoding genes and prfB. |
Information sourced from Uniprot.org
