STJ70614 staining (1ยตg/ml) of human brain lysate (RIPA buffer, 35ยตg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Anti-RIC8A/Synembryn antibody (C-Term) (STJ70614)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
STJ70614-100
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General Information

Short DescriptionGoat polyclonal antibody anti-RIC8A/Synembryn (C-Term) is suitable for use in ELISA and Western Blot research applications.
ApplicationsPep-ELISA/WB
HostGoat
ReactivityHuman/Mouse/Rat/Cow
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
Concentration0.5 mg/mL
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution RangePeptide ELISA: antibody detection limit dilution 1:32000.
WB: Approx 70kDa band observed in human brain lysates (calculated MW of 60.4kDa according to NP_068751.4). Recommended concentration: 1-3ยตg/ml.
Formulation0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Storage InstructionStore at-20ยฐC on receipt and minimise freeze-thaw cycles.

Target Information

Gene SymbolRIC8A
Gene ID60626
Uniprot IDRIC8A_HUMAN
Accession NumberNP_068751.4
Immunogen RegionC-Term
Immunogen SequenceCLSSDPDSDPD

Additional Info

Function Chaperone that specifically binds and folds nascent G alpha proteins prior to G protein heterotrimer formation, promoting their stability and activity: folds GNAI1, GNAO1, GNA13 and GNAQ. Does not fold G(s) G-alpha proteins GNAS nor GNAL. Also acts as a guanine nucleotide exchange factor (GEF) for G alpha proteins by stimulating exchange of bound GDP for free GTP. Involved in regulation of microtubule pulling forces during mitotic movement of chromosomes by stimulating G(i)-alpha protein (GNAI1), possibly leading to release G(i)-alpha-GTP and NuMA proteins from the NuMA-GPSM2-G(i)-alpha-GDP complex. Also acts as an activator for G(q)-alpha (GNAQ) protein by enhancing the G(q)-coupled receptor-mediated ERK activation.
Protein Name Chaperone Ric-8a
Synembryn-A
Cellular Localisation Cytoplasm
Cell Cortex
Alternative Antibody Names Anti-Chaperone Ric-8a antibody
Anti-Synembryn-A antibody
Anti-RIC8A antibody

Information sourced from Uniprot.org

Citations

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