• Immunofluorescence analysis of PC-12 cells using RhoC Rabbit polyclonal antibody (STJ11104547) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using RhoC Rabbit polyclonal antibody (STJ11104547) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from Rat heart, using RhoC Rabbit polyclonal antibody (STJ11104547) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-RHOC antibody (94-193) (STJ11104547)

SKU:
STJ11104547

£112.50 - £522.50
Current Stock:
Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Rho-Related Gtp-Binding Protein Rhoc (94-193) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RHOC
Gene ID: 389
Uniprot ID: RHOC_HUMAN
Immunogen Region: 94-193
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 94-193 of human RhoC (NP_786886.1).
Immunogen Sequence: NIPEKWTPEVKHFCPNVPII LVGNKKDLRQDEHTRRELAK MKQEPVRSEEGRDMANRISA FGYLECSAKTKEGVREVFEM ATRAGLQVRKNKRRRGCPIL
Post Translational Modifications (Microbial infection) Glycosylated at Tyr-34 by Photorhabdus asymbiotica toxin PAU_02230. Mono-O-GlcNAcylation by PAU_02230 inhibits downstream signaling by an impaired interaction with diverse regulator and effector proteins of Rho and leads to actin disassembly. (Microbial infection) Glucosylated at Thr-37 by C.difficile toxins TcdA and TcdB in the colonic epithelium. Monoglucosylation completely prevents the recognition of the downstream effector, blocking the GTPases in their inactive form, leading to actin cytoskeleton disruption.
Function Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Serves as a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Regulates apical junction formation in bronchial epithelial cells.
Protein Name Rho-Related Gtp-Binding Protein Rhoc
Rho Cdna Clone 9
H9
Database Links Reactome: R-HSA-416482
Reactome: R-HSA-416572
Reactome: R-HSA-5625740
Reactome: R-HSA-5625900
Reactome: R-HSA-5627117
Reactome: R-HSA-5663220
Reactome: R-HSA-5666185
Reactome: R-HSA-9013106
Cellular Localisation Cell Membrane
Lipid-Anchor
Cytoplasmic Side
Cleavage Furrow
Translocates To The Equatorial Region Before Furrow Formation In A Ect2-Dependent Manner
Alternative Antibody Names Anti-Rho-Related Gtp-Binding Protein Rhoc antibody
Anti-Rho Cdna Clone 9 antibody
Anti-H9 antibody
Anti-RHOC antibody
Anti-ARH9 antibody
Anti-ARHC antibody

Information sourced from Uniprot.org

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