Anti-RHOB antibody (99-148 aa) (STJ95443)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human RHOB at the amino acid range 99-148
General Information
| Short Description | Rabbit polyclonal anti-Rho-related GTP-binding protein RhoB (99-148 aa) for use in WB, ELISA and IHC in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA/IHC |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000IHC-P 1:50-300ELISA 2000-20000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | RHOB |
| Gene ID | 388 |
| Uniprot ID | RHOB_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human RHOB at the amino acid range 99-148 |
| Immunogen Region | 99-148 aa |
| Specificity | Rho B Polyclonal Antibody detects endogenous levels of Rho B protein. |
Additional Info
| Function | Mediates apoptosis in neoplastically transformed cells after DNA damage. Not essential for development but affects cell adhesion and growth factor signaling in transformed cells. Plays a negative role in tumorigenesis as deletion causes tumor formation. Involved in intracellular protein trafficking of a number of proteins. Targets PKN1 to endosomes and is involved in trafficking of the EGF receptor from late endosomes to lysosomes. Also required for stability and nuclear trafficking of AKT1/AKT which promotes endothelial cell survival during vascular development. Serves as a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Required for genotoxic stress-induced cell death in breast cancer cells. |
| Protein Name | Rho-Related Gtp-Binding Protein RhobRho Cdna Clone 6H6 |
| Database Links | Reactome: R-HSA-114604Reactome: R-HSA-416482Reactome: R-HSA-416572Reactome: R-HSA-5625740Reactome: R-HSA-5625900Reactome: R-HSA-5627117Reactome: R-HSA-5663220Reactome: R-HSA-5666185Reactome: R-HSA-9013026 |
| Cellular Localisation | Late Endosome MembraneLipid-AnchorCell MembraneNucleusCleavage FurrowLate Endosomal Membrane (Geranylgeranylated Form)Plasma Membrane (Farnesylated Form)Also Detected At The Nuclear Margin And In The NucleusTranslocates To The Equatorial Region Before Furrow Formation In A Ect2-Dependent Manner |
| Alternative Antibody Names | Anti-Rho-Related Gtp-Binding Protein Rhob antibodyAnti-Rho Cdna Clone 6 antibodyAnti-H6 antibodyAnti-RHOB antibodyAnti-ARH6 antibodyAnti-ARHB antibody |
Information sourced from Uniprot.org