| Host: | Rabbit |
| Applications: | WB/ELISA/IHC |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-Rho-related GTP-binding protein RhoB (99-148 aa) is suitable for use in Western Blot, ELISA and Immunohistochemistry research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-2000IHC-P 1:50-300ELISA 2000-20000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | RHOB |
| Gene ID: | 388 |
| Uniprot ID: | RHOB_HUMAN |
| Immunogen Region: | 99-148 aa |
| Specificity: | Rho B Polyclonal Antibody detects endogenous levels of Rho B protein. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from the human RHOB at the amino acid range 99-148 |
| Function | Mediates apoptosis in neoplastically transformed cells after DNA damage. Not essential for development but affects cell adhesion and growth factor signaling in transformed cells. Plays a negative role in tumorigenesis as deletion causes tumor formation. Involved in intracellular protein trafficking of a number of proteins. Targets PKN1 to endosomes and is involved in trafficking of the EGF receptor from late endosomes to lysosomes. Also required for stability and nuclear trafficking of AKT1/AKT which promotes endothelial cell survival during vascular development. Serves as a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Required for genotoxic stress-induced cell death in breast cancer cells. |
| Protein Name | Rho-Related Gtp-Binding Protein RhobRho Cdna Clone 6H6 |
| Database Links | Reactome: R-HSA-114604Reactome: R-HSA-416482Reactome: R-HSA-416572Reactome: R-HSA-5625740Reactome: R-HSA-5625900Reactome: R-HSA-5627117Reactome: R-HSA-5663220Reactome: R-HSA-5666185Reactome: R-HSA-9013026 |
| Cellular Localisation | Late Endosome MembraneLipid-AnchorCell MembraneNucleusCleavage FurrowLate Endosomal Membrane (Geranylgeranylated Form)Plasma Membrane (Farnesylated Form)Also Detected At The Nuclear Margin And In The NucleusTranslocates To The Equatorial Region Before Furrow Formation In A Ect2-Dependent Manner |
| Alternative Antibody Names | Anti-Rho-Related Gtp-Binding Protein Rhob antibodyAnti-Rho Cdna Clone 6 antibodyAnti-H6 antibodyAnti-RHOB antibodyAnti-ARH6 antibodyAnti-ARHB antibody |
Information sourced from Uniprot.org
