• Immunofluorescence analysis of NIH-3T3 cells using RHOA/RHOB/RHOC Rabbit monoclonal antibody (STJ11102169) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded rat testis using RHOA/RHOB/RHOC Rabbit monoclonal antibody (STJ11102169) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using RHOA/RHOB/RHOC Rabbit monoclonal antibody (STJ11102169) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-RHOA/RHOB/RHOC antibody (1-193) [S9MR] (STJ11102169)

SKU:
STJ11102169

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Host: Rabbit
Applications: WB/IHC-P/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Monoclonal
Clone ID: S9MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IHC-P:1:50-1:200
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RHOC
RHOB
389
388
387
RHOC_HUMAN
RHOB_HUMAN
RHOA_HUMAN
Immunogen Region: 1-193
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-193 of human RHOA/RHOB/RHOC (P08134).
Immunogen Sequence: MAAIRKKLVIVGDGACGKTC LLIVFSKDQFPEVYVPTVFE NYIADIEVDGKQVELALWDT AGQEDYDRLRPLSYPDTDVI LMCFSIDSPDSLENIPEKWT PEVKHFCPNVPIILVGNKKD LRQDEHTRRELAKMKQEPVR SEEGRDMANRISAFGYLECS AKTKEGVREVFEMATRAGLQ VRKNKRRRGCPIL
Tissue Specificity
Alternative Antibody Names Anti-Rho-Related Gtp-Binding Protein Rhoc antibody
Anti-Rho Cdna Clone 9 antibody
Anti-H9 antibody
Anti-RHOC antibody
Anti-ARH9 antibody
Anti-ARHC antibody
Anti-Rho-Related Gtp-Binding Protein Rhob antibody
Anti-Rho Cdna Clone 6 antibody
Anti-H6 antibody
Anti-RHOB antibody
Anti-ARH6 antibody
Anti-ARHB antibody
Anti-Transforming Protein Rhoa antibody
Anti-Rho Cdna Clone 12 antibody
Anti-H12 antibody
Anti-RHOA antibody
Anti-ARH12 antibody
Anti-ARHA antibody
Anti-RHO12 antibody
Anti-Rho-Related Gtp-Binding Protein Rhoc antibody
Anti-Rho Cdna Clone 9 antibody
Anti-H9 antibody
Anti-RHOC antibody
Anti-ARH9 antibody
Anti-ARHC antibody
Anti-Rho-Related Gtp-Binding Protein Rhob antibody
Anti-Rho Cdna Clone 6 antibody
Anti-H6 antibody
Anti-RHOB antibody
Anti-ARH6 antibody
Anti-ARHB antibody
Anti-Transforming Protein Rhoa antibody
Anti-Rho Cdna Clone 12 antibody
Anti-H12 antibody
Anti-RHOA antibody
Anti-ARH12 antibody
Anti-ARHA antibody
Anti-RHO12 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance