• Western blot analysis of lysates from HepG2 cells, using RhoA Antibody. The lane on the right is blocked with the synthesized peptide.
  • Western blot analysis of various cells using Rho A Polyclonal Antibody diluted at 1：500
  • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using RhoA Antibody. The picture on the right is blocked with the synthesized peptide.
  • Immunohistochemical analysis of paraffin-embedded Mouse-lung tissue. 1, Rho A Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue. 1, Rho A Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1, Rho A Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1, Rho A Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Human-stomach-cancer tissue. 1, Rho A Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Rat-liver tissue. 1, Rho A Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunofluorescence analysis of mouse-lung tissue. 1, Rho A Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Immunofluorescence analysis of mouse-lung tissue. 1, Rho A Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Immunofluorescence analysis of rat-lung tissue. 1, Rho A Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Immunofluorescence analysis of rat-lung tissue. 1, Rho A Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Immunofluorescence analysis of Hela cell. 1, Rho A Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

Anti-RHOA antibody (144-193 aa) (STJ95442)

SKU:
STJ95442

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: IF/WB/IHC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Transforming protein RhoA (144-193 aa) is suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: IF 1:50-200
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:5000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RHOA
Gene ID: 387
Uniprot ID: RHOA_HUMAN
Immunogen Region: 144-193 aa
Specificity: Rho A Polyclonal Antibody detects endogenous levels of Rho A protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human RhoA at the amino acid range 144-193
Function Small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. Mainly associated with cytoskeleton organization, in active state binds to a variety of effector proteins to regulate cellular responses such as cytoskeletal dynamics, cell migration and cell cycle. Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Involved in a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Plays an essential role in cleavage furrow formation. Required for the apical junction formation of keratinocyte cell-cell adhesion. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization. Regulates KCNA2 potassium channel activity by reducing its location at the cell surface in response to CHRM1 activation.promotes KCNA2 endocytosis. Acts as an allosteric activator of guanine nucleotide exchange factor ECT2 by binding in its activated GTP-bound form to the PH domain of ECT2 which stimulates the release of PH inhibition and promotes the binding of substrate RHOA to the ECT2 catalytic center. May be an activator of PLCE1. In neurons, involved in the inhibition of the initial spine growth. Upon activation by CaMKII, modulates dendritic spine structural plasticity by relaying CaMKII transient activation to synapse-specific, long-term signaling. Acts as a regulator of platelet alpha-granule release during activation and aggregation of platelets. (Microbial infection) Serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague.
Protein Name Transforming Protein Rhoa
Rho Cdna Clone 12
H12
Database Links Reactome: R-HSA-114604
Reactome: R-HSA-193634
Reactome: R-HSA-198203
Reactome: R-HSA-209563
Reactome: R-HSA-2173791
Reactome: R-HSA-392451
Reactome: R-HSA-3928662
Reactome: R-HSA-3928663
Reactome: R-HSA-4086400
Reactome: R-HSA-416482
Reactome: R-HSA-416550
Reactome: R-HSA-416572
Reactome: R-HSA-4420097
Reactome: R-HSA-5625740
Reactome: R-HSA-5625900
Reactome: R-HSA-5625970
Reactome: R-HSA-5627117
Reactome: R-HSA-5663220
Reactome: R-HSA-5666185
Reactome: R-HSA-5689896
Reactome: R-HSA-6785631
Reactome: R-HSA-6798695
Reactome: R-HSA-8849471
Reactome: R-HSA-8980692
Reactome: R-HSA-8985586
Reactome: R-HSA-9013106
Cellular Localisation Cell Membrane
Lipid-Anchor
Cytoplasmic Side
Cytoplasm
Cytoskeleton
Cleavage Furrow
Cell Cortex
Midbody
Cell Projection
Lamellipodium
Dendrite
Nucleus
Localized To Cell-Cell Contacts In Calcium-Treated Keratinocytes
Translocates To The Equatorial Region Before Furrow Formation In A Ect2-Dependent Manner
Localizes To The Equatorial Cell Cortex (At The Site Of The Presumptive Furrow) In Early Anaphase In An Activated Form And In A Myosin- And Actin-Independent Manner
Alternative Antibody Names Anti-Transforming Protein Rhoa antibody
Anti-Rho Cdna Clone 12 antibody
Anti-H12 antibody
Anti-RHOA antibody
Anti-ARH12 antibody
Anti-ARHA antibody
Anti-RHO12 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance