• Immunohistochemistry analysis of paraffin-embedded human brain tissue, using Rhodopsin Antibody. The picture on the right is blocked with the synthesized peptide.
  • Western blot analysis of lysates from COLO cells, using Rhodopsin Antibody. The lane on the right is blocked with the synthesized peptide.
  • Western blot analysis of various cells using Rhodopsin Polyclonal Antibody

Anti-RHO antibody (299-348 aa) (STJ95452)

SKU:
STJ95452

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Rhodopsin (299-348 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:5000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RHO
Gene ID: 6010
Uniprot ID: OPSD_HUMAN
Immunogen Region: 299-348 aa
Specificity: Rhodopsin Polyclonal Antibody detects endogenous levels of Rhodopsin protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human Rhodopsin at the amino acid range 299-348
Post Translational Modifications Phosphorylated on some or all of the serine and threonine residues present in the C-terminal region. After activation by light, phosphorylated by GRK1 (in vitro). Contains one covalently linked retinal chromophore. Upon light absorption, the covalently bound 11-cis-retinal is converted to all-trans-retinal. After hydrolysis of the Schiff base and release of the covalently bound all-trans-retinal, active rhodopsin is regenerated by binding of a fresh molecule of 11-cis-retinal.
Function Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth. Light-induced isomerization of the chromophore 11-cis-retinal to all-trans-retinal triggers a conformational change that activates signaling via G-proteins. Subsequent receptor phosphorylation mediates displacement of the bound G-protein alpha subunit by the arrestin SAG and terminates signaling.
Protein Name Rhodopsin
Opsin-2
Database Links Reactome: R-HSA-2453902
Reactome: R-HSA-2485179
Reactome: R-HSA-2514859
Reactome: R-HSA-418594
Reactome: R-HSA-419771
Reactome: R-HSA-5620916
Cellular Localisation Membrane
Multi-Pass Membrane Protein
Cell Projection
Cilium
Photoreceptor Outer Segment
Synthesized In The Inner Segment (Is) Of Rod Photoreceptor Cells Before Vectorial Transport To Disk Membranes In The Rod Outer Segment (Os) Photosensory Cilia
Alternative Antibody Names Anti-Rhodopsin antibody
Anti-Opsin-2 antibody
Anti-RHO antibody
Anti-OPN2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance