Anti-RHEB antibody (1-180) (STJ25350)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal RHEB (1-180) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | RHEB |
| Gene ID | 6009 |
| Uniprot ID | RHEB_HUMAN |
| Immunogen Region | 1-180 |
| Immunogen Sequence | MPQSKSRKIAILGYRSVGKS SLTIQFVEGQFVDSYDPTIE NTFTKLITVNGQEYHLQLVD TAGQDEYSIFPQTYSIDING YILVYSVTSIKSFEVIKVIH GKLLDMVGKVQIPIMLVGNK KDLHMERVISYEEGKALAES WNAAFLESSAKENQTAVDVF RRIILEAEKMDGAASQGKSS |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human RheB (NP_005605.1). |
Additional Info
| Tissue Specificity | Ubiquitous. Highest levels observed in skeletal and cardiac muscle. |
| Post Translational Modifications | Farnesylation is important for efficiently activating mTORC1-mediated signaling. Polyubiquitinated in response to amino acid, promoting its interaction with MTOR and mTORC1 activation. Deubiquitination by ATXN3 promotes recruitment of the TSC-TBC complex and RHEB inactivation by TSC2. Monoubiquitinated at Lys-8 by RNF152, promoting its association with the TSC-TBC complex. Deubiquitinated at Lys-8 by USP4, promoting mTORC1 activation. Phosphorylation by MAPKAPK5 impairs GTP-binding and inactivation. |
| Function | Small GTPase that acts as an allosteric activator of the canonical mTORC1 complex, an evolutionarily conserved central nutrient sensor that stimulates anabolic reactions and macromolecule biosynthesis to promote cellular biomass generation and growth. In response to nutrients, growth factors or amino acids, specifically activates the protein kinase activity of MTOR, the catalytic component of the mTORC1 complex: acts by causing a conformational change that allows the alignment of residues in the active site of MTOR, thereby enhancing the phosphorylation of ribosomal protein S6 kinase (RPS6KB1 and RPS6KB2) and EIF4EBP1 (4E-BP1). RHEB is also required for localization of the TSC-TBC complex to lysosomal membranes. In response to starvation, RHEB is inactivated by the TSC-TBC complex, preventing activation of mTORC1. Has low intrinsic GTPase activity. |
| Protein Name | Gtp-Binding Protein RhebRas Homolog Enriched In Brain |
| Database Links | Reactome: R-HSA-1632852Reactome: R-HSA-165159Reactome: R-HSA-166208Reactome: R-HSA-380972Reactome: R-HSA-5628897Reactome: R-HSA-8943724Reactome: R-HSA-9639288 |
| Cellular Localisation | Endomembrane SystemLipid-AnchorCytoplasmic SideLysosome MembraneGolgi Apparatus MembraneEndoplasmic Reticulum MembraneCytoplasmCytosolFarnesylation Is Required For Recruitment To Lysosomal MembranesWhere It Activates The Mtorc1 Complex |
| Alternative Antibody Names | Anti-Gtp-Binding Protein Rheb antibodyAnti-Ras Homolog Enriched In Brain antibodyAnti-RHEB antibodyAnti-RHEB2 antibody |
Information sourced from Uniprot.org