Anti-RFWD3 antibody (374-423 aa) (STJ95429)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human RFWD3 at the amino acid range 374-423
General Information
| Short Description | Rabbit polyclonal anti-E3 ubiquitin-protein ligase RFWD3 (374-423 aa) for use in WB, IHC, IF and ELISA in Human, Rat and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Rat/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC 1:100-1:300ELISA 1:40000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | RFWD3 |
| Gene ID | 55159 |
| Uniprot ID | RFWD3_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human RFWD3 at the amino acid range 374-423 |
| Immunogen Region | 374-423 aa |
| Specificity | RFWD3 Polyclonal Antibody detects endogenous levels of RFWD3 protein. |
Additional Info
| Function | E3 ubiquitin-protein ligase required for the repair of DNA interstrand cross-links (ICL) in response to DNA damage. Plays a key role in RPA-mediated DNA damage signaling and repair. Acts by mediating ubiquitination of the RPA complex (RPA1, RPA2 and RPA3 subunits) and RAD51 at stalled replication forks, leading to remove them from DNA damage sites and promote homologous recombination. Also mediates the ubiquitination of p53/TP53 in the late response to DNA damage, and acts as a positive regulator of p53/TP53 stability, thereby regulating the G1/S DNA damage checkpoint. May act by catalyzing the formation of short polyubiquitin chains on p53/TP53 that are not targeted to the proteasome. In response to ionizing radiation, interacts with MDM2 and enhances p53/TP53 ubiquitination, possibly by restricting MDM2 from extending polyubiquitin chains on ubiquitinated p53/TP53. Required to translesion DNA synthesis across DNA-protein cross-link adducts by catalyzing ubiquitination of proteins on single-stranded DNA (ssDNA). |
| Protein Name | E3 Ubiquitin-Protein Ligase Rfwd3Ring Finger And Wd Repeat Domain-Containing Protein 3Ring Finger Protein 201 |
| Database Links | |
| Cellular Localisation | NucleusPml BodyCytoplasmIn Undamaged CellsFound Both In The Cytoplasm And In The NucleusPartially Associated With Pml Nuclear BodiesIn Response To Replication BlockSuch As That Caused By Hydroxyurea TreatmentOr To Dna Damage Caused By Ionizing Radiations Or DoxorubicinRecruited To The NucleusTo Stalled Replication Forks Or To Sites Of Dna RepairThis Recruitment Depends Upon Rpa2 |
| Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Rfwd3 antibodyAnti-Ring Finger And Wd Repeat Domain-Containing Protein 3 antibodyAnti-Ring Finger Protein 201 antibodyAnti-RFWD3 antibodyAnti-RNF201 antibody |
Information sourced from Uniprot.org