• Western blot analysis of lysates from MCF-7 cells, using RFWD3 Antibody. The lane on the right is blocked with the synthesized peptide.
  • Western blot analysis of the lysates from HepG2 cells using RFWD3 antibody.
  • Western blot analysis of 823 cells using RFWD3 Polyclonal Antibody diluted at 1：2000

Anti-RFWD3 antibody (374-423 aa) (STJ95429)

SKU:
STJ95429

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Rat/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-E3 ubiquitin-protein ligase RFWD3 (374-423 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:40000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RFWD3
Gene ID: 55159
Uniprot ID: RFWD3_HUMAN
Immunogen Region: 374-423 aa
Specificity: RFWD3 Polyclonal Antibody detects endogenous levels of RFWD3 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human RFWD3 at the amino acid range 374-423
Function E3 ubiquitin-protein ligase required for the repair of DNA interstrand cross-links (ICL) in response to DNA damage. Plays a key role in RPA-mediated DNA damage signaling and repair. Acts by mediating ubiquitination of the RPA complex (RPA1, RPA2 and RPA3 subunits) and RAD51 at stalled replication forks, leading to remove them from DNA damage sites and promote homologous recombination. Also mediates the ubiquitination of p53/TP53 in the late response to DNA damage, and acts as a positive regulator of p53/TP53 stability, thereby regulating the G1/S DNA damage checkpoint. May act by catalyzing the formation of short polyubiquitin chains on p53/TP53 that are not targeted to the proteasome. In response to ionizing radiation, interacts with MDM2 and enhances p53/TP53 ubiquitination, possibly by restricting MDM2 from extending polyubiquitin chains on ubiquitinated p53/TP53.
Protein Name E3 Ubiquitin-Protein Ligase Rfwd3
Ring Finger And Wd Repeat Domain-Containing Protein 3
Ring Finger Protein 201
Cellular Localisation Nucleus
Pml Body
Cytoplasm
In Undamaged Cells
Found Both In The Cytoplasm And In The Nucleus
Partially Associated With Pml Nuclear Bodies
In Response To Replication Block
Such As That Caused By Hydroxyurea Treatment
Or To Dna Damage Caused By Ionizing Radiations Or Doxorubicin
Recruited To The Nucleus
To Stalled Replication Forks Or To Sites Of Dna Repair
This Recruitment Depends Upon Rpa2
Alternative Antibody Names Anti-E3 Ubiquitin-Protein Ligase Rfwd3 antibody
Anti-Ring Finger And Wd Repeat Domain-Containing Protein 3 antibody
Anti-Ring Finger Protein 201 antibody
Anti-RFWD3 antibody
Anti-RNF201 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance