• Anti-RFP antibody conjugated to DyLight® 550 at 1/2, 500 dilution using HEK293 transfected cell lysates at 50 µg per lane
  • Immunofluorescence – anti-RFP antibody conjugated to DyLight® 550 using COS-7 cells transduced with GFP-Rab5a (signal amplification) ; cells were fixed with methanol and anti-RFP at 1/250

Anti-RFP antibody {DyLight®550} (STJ140209)

SKU:
STJ140209

£141.50 - £425.50
Current Stock:
Host: Goat
Applications: WB/IHC-F/IF
Reactivity: RFP/mCherry/mOrange/mPlum/mRFP/mStrawberry/tdTomato
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-RFP is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: DyLight®550
Isotype: IgG
Formulation: PBS, 20% Glycerol, 0.05% ProClin® and 0.05% Sodium Azide.
Purification: Epitope affinity purified
Concentration: 2 mg/ml
Dilution Range: WB 1:500-1:2000
IHC-F 1:50-1:1000
IF 1:50-1:500
Storage Instruction: For continuous use, store at 2-8 C for one-two days. For extended storage, store in-20 C freezer. Working dilution samples should be discarded if not used within 12 hours.
Specificity: In 293HEK cells transfected with cds plasmid detects a band of 29 kDa by Western blot. This antibody also recognizes very well tdTomato and does not cross-react to GFP (green fluorescent protein).
Immunogen: Purified recombinant RFP peptide produced in E. coli.
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance