Anti-Recombinant-TRIM56 antibody [RM2N95] (STJA0026257)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal anti-Recombinant-E3 Ubiquitin-Protein Ligase Trim56 for use in IF and WB in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IF/WB |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | RM2N95 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Protein A/G purified from cell culture supernatant |
| Dilution Range | IF: 1:50-1:200, WB: 1:500-1:1000 |
| Formulation | 0.01M PBS, pH 7.4, 0.05% BSA, 50% Glycerol, 0.05% Sodium Azide |
| Storage Instruction | Suitable for storage at +4°C between 1-2 weeks. For longer term store at-20°C for up to 12 months. |
Target Information
| Gene Symbol | TRIM56 |
| Gene ID | 81844 |
| Uniprot ID | TRI56_HUMAN |
Additional Info
| Post Translational Modifications | (Microbial infection) Preferentially ubiquitinated with 'Lys-48' and 'Lys-11'-linked ubiquitin chains by Salmonella effector SopA leading to proteasomal targeting and degradation. Autoubiquitinated. |
| Function | E3 ubiquitin-protein ligase that plays a key role in innate antiviral immunity by mediating ubiquitination of CGAS and STING1. In response to pathogen- and host-derived double-stranded DNA (dsDNA), targets STING1 to 'Lys-63'-linked ubiquitination, thereby promoting its homodimerization, a step required for the production of type I interferon IFN-beta. Also mediate monoubiquitination of CGAS, thereby promoting CGAS oligomerization and subsequent activation. Promotes also TNFalpha-induced NF-kappa-B signaling by mediating 'Lys-63'-linked ubiquitination TAK1, leading to enhanced interaction between TAK1 and CHUK/IKKalpha. Independently of its E3 ubiquitin ligase activity, positive regulator of TLR3 signaling. Potentiates extracellular double stranded RNA (dsRNA)-induced expression of IFNB1 and interferon-stimulated genes ISG15, IFIT1/ISG56, CXCL10, OASL and CCL5/RANTES. Promotes establishment of an antiviral state by TLR3 ligand and TLR3-mediated chemokine induction following infection by hepatitis C virus. Acts as a restriction factor of Zika virus through direct interaction with the viral RNA via its C-terminal region. |
| Protein Name | E3 Ubiquitin-Protein Ligase Trim56Ring Finger Protein 109Tripartite Motif-Containing Protein 56 |
| Database Links | Reactome: R-HSA-3134975 |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Trim56 antibodyAnti-Ring Finger Protein 109 antibodyAnti-Tripartite Motif-Containing Protein 56 antibodyAnti-TRIM56 antibodyAnti-RNF109 antibody |
Information sourced from Uniprot.org