Anti-Recombinant-SRSF1 antibody [RM2D18] (STJA0025289)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal anti-Recombinant-Serine/Arginine-Rich Splicing Factor 1 for use in IF, IHC and WB in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IF/IHC/WB |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | RM2D18 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Protein A/G purified from cell culture supernatant |
| Dilution Range | IF: 1:50-1:200, IHC: 1:50-1:100, WB: 1:500-1:1000 |
| Formulation | 0.01M PBS, pH 7.4, 0.05% BSA, 50% Glycerol, 0.05% Sodium Azide |
| Storage Instruction | Suitable for storage at +4°C between 1-2 weeks. For longer term store at-20°C for up to 12 months. |
Target Information
| Gene Symbol | SRSF1 |
| Gene ID | 6426 |
| Uniprot ID | SRSF1_HUMAN |
Additional Info
| Function | Plays a role in preventing exon skipping, ensuring the accuracy of splicing and regulating alternative splicing. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Can stimulate binding of U1 snRNP to a 5'-splice site-containing pre-mRNA. Binds to purine-rich RNA sequences, either the octamer, 5'-RGAAGAAC-3' (r=A or G) or the decamers, AGGACAGAGC/AGGACGAAGC. Binds preferentially to the 5'-CGAGGCG-3' motif in vitro. Three copies of the octamer constitute a powerful splicing enhancer in vitro, the ASF/SF2 splicing enhancer (ASE) which can specifically activate ASE-dependent splicing. Isoform ASF-2 and isoform ASF-3 act as splicing repressors. May function as export adapter involved in mRNA nuclear export through the TAP/NXF1 pathway. |
| Protein Name | Serine/Arginine-Rich Splicing Factor 1Alternative-Splicing Factor 1Asf-1Splicing Factor - Arginine/Serine-Rich 1Pre-Mrna-Splicing Factor Sf2 - P33 Subunit |
| Database Links | Reactome: R-HSA-159236Reactome: R-HSA-72163Reactome: R-HSA-72165Reactome: R-HSA-72187Reactome: R-HSA-72203Reactome: R-HSA-73856 |
| Cellular Localisation | CytoplasmNucleus SpeckleIn Nuclear SpecklesShuttles Between The Nucleus And The CytoplasmNuclear Import Is Mediated Via Interaction With Tnpo3 |
| Alternative Antibody Names | Anti-Serine/Arginine-Rich Splicing Factor 1 antibodyAnti-Alternative-Splicing Factor 1 antibodyAnti-Asf-1 antibodyAnti-Splicing Factor - Arginine/Serine-Rich 1 antibodyAnti-Pre-Mrna-Splicing Factor Sf2 - P33 Subunit antibodyAnti-SRSF1 antibodyAnti-ASF antibodyAnti-SF2 antibodyAnti-SF2P33 antibodyAnti-SFRS1 antibodyAnti-OK antibodyAnti-SW-cl.3 antibody |
Information sourced from Uniprot.org