Anti-Recombinant-SMAD4 antibody [RM2E42] (STJA0025412)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal anti-Recombinant-Mothers Against Decapentaplegic Homolog 4 for use in IF, IP and WB in Human and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IF/IP/WB |
| Host | Rabbit |
| Reactivity | Human/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | RM2E42 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Protein A/G purified from cell culture supernatant |
| Dilution Range | IF: 1:50-1:200, IP: 1:20, WB: 1:500-1:1000 |
| Formulation | 0.01M PBS, pH 7.4, 0.05% BSA, 50% Glycerol, 0.05% Sodium Azide |
| Storage Instruction | Suitable for storage at +4°C between 1-2 weeks. For longer term store at-20°C for up to 12 months. |
Target Information
| Gene Symbol | SMAD4 |
| Gene ID | 4089 |
| Uniprot ID | SMAD4_HUMAN |
Additional Info
| Function | In muscle physiology, plays a central role in the balance between atrophy and hypertrophy. When recruited by MSTN, promotes atrophy response via phosphorylated SMAD2/4. MSTN decrease causes SMAD4 release and subsequent recruitment by the BMP pathway to promote hypertrophy via phosphorylated SMAD1/5/8. Acts synergistically with SMAD1 and YY1 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression. Binds to SMAD binding elements (SBEs) (5'-GTCT/AGAC-3') within BMP response element (BMPRE) of cardiac activating regions. Common SMAD (co-SMAD) is the coactivator and mediator of signal transduction by TGF-beta (transforming growth factor). Component of the heterotrimeric SMAD2/SMAD3-SMAD4 complex that forms in the nucleus and is required for the TGF-mediated signaling. Promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. Component of the multimeric SMAD3/SMAD4/JUN/FOS complex which forms at the AP1 promoter site.required for synergistic transcriptional activity in response to TGF-beta. May act as a tumor suppressor. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. |
| Protein Name | Mothers Against Decapentaplegic Homolog 4Mad Homolog 4Mothers Against Dpp Homolog 4Deletion Target In Pancreatic Carcinoma 4Smad Family Member 4Smad 4Smad4Hsmad4 |
| Database Links | Reactome: R-HSA-1181150Reactome: R-HSA-1502540Reactome: R-HSA-201451Reactome: R-HSA-2173789Reactome: R-HSA-2173795Reactome: R-HSA-2173796Reactome: R-HSA-3311021Reactome: R-HSA-3315487Reactome: R-HSA-452723Reactome: R-HSA-5689880Reactome: R-HSA-8941326Reactome: R-HSA-8941855Reactome: R-HSA-8952158Reactome: R-HSA-9615017Reactome: R-HSA-9617828Reactome: R-HSA-9733709Reactome: R-HSA-9735871Reactome: R-HSA-9754189Reactome: R-HSA-9823730Reactome: R-HSA-9839394Reactome: R-HSA-9844594 |
| Cellular Localisation | CytoplasmNucleusCytoplasmic In The Absence Of LigandMigrates To The Nucleus When Complexed With R-SmadPdpk1 Prevents Its Nuclear Translocation In Response To Tgf-Beta |
| Alternative Antibody Names | Anti-Mothers Against Decapentaplegic Homolog 4 antibodyAnti-Mad Homolog 4 antibodyAnti-Mothers Against Dpp Homolog 4 antibodyAnti-Deletion Target In Pancreatic Carcinoma 4 antibodyAnti-Smad Family Member 4 antibodyAnti-Smad 4 antibodyAnti-Smad4 antibodyAnti-Hsmad4 antibodyAnti-SMAD4 antibodyAnti-DPC4 antibodyAnti-MADH4 antibody |
Information sourced from Uniprot.org