| Post Translational Modifications | Phosphorylated by PRKDC at the C-terminus in response to DNA damage. Phosphorylations by PRKDC at the C-terminus of XRCC4 and NHEJ1/XLF are highly redundant and regulate ability of the XRCC4-NHEJ1/XLF subcomplex to bridge DNA. Phosphorylation does not prevent interaction with XRCC4 but disrupts ability to bridge DNA and promotes detachment from DNA. |
| Function | DNA repair protein involved in DNA non-homologous end joining (NHEJ).required for double-strand break (DSB) repair and V(D)J recombination. Plays a key role in NHEJ by promoting the ligation of various mismatched and non-cohesive ends. Together with PAXX, collaborates with DNA polymerase lambda (POLL) to promote joining of non-cohesive DNA ends. May act in concert with XRCC5-XRCC6 (Ku) to stimulate XRCC4-mediated joining of blunt ends and several types of mismatched ends that are non-complementary or partially complementary. In some studies, has been shown to associate with XRCC4 to form alternating helical filaments that bridge DNA and act like a bandage, holding together the broken DNA until it is repaired. Alternatively, it has also been shown that rather than forming filaments, a single NHEJ1 dimer interacts through both head domains with XRCC4 to promote the close alignment of DNA ends. The XRCC4-NHEJ1/XLF subcomplex binds to the DNA fragments of a DSB in a highly diffusive manner and robustly bridges two independent DNA molecules, holding the broken DNA fragments in close proximity to one other. The mobility of the bridges ensures that the ends remain accessible for further processing by other repair factors. Binds DNA in a length-dependent manner. |
| Protein Name | Non-Homologous End-Joining Factor 1Protein CernunnosXrcc4-Like Factor |
| Database Links | Reactome: R-HSA-5693571 |
| Cellular Localisation | NucleusChromosomeLocalizes To Site Of Double-Strand BreaksRecruitment Is Dependent On Xrcc5-Xrcc6 (Ku) Heterodimer |
| Alternative Antibody Names | Anti-Non-Homologous End-Joining Factor 1 antibodyAnti-Protein Cernunnos antibodyAnti-Xrcc4-Like Factor antibodyAnti-NHEJ1 antibodyAnti-XLF antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and NHEJ1 knockout (KO) HeLa cells, using [KO Validated] NHEJ1 Rabbit polyclonal antibody (STJ11103937) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/176582/367429/STJ11103937_1__73085.1713132967.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and NHEJ1 knockout (KO) HeLa cells, using [KO Validated] NHEJ1 Rabbit polyclonal antibody (STJ11103937) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.")
