| Host: | Rabbit |
| Applications: | IP/WB |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit monoclonal antibody anti-Recombinant-Inositol-Tetrakisphosphate 1-Kinase is suitable for use in Immunoprecipitation and Western Blot research applications. |
| Clonality : | Monoclonal |
| Clone ID : | RM2E64 |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.01M PBS, pH 7.4, 0.05% BSA, 50% Glycerol, 0.05% Sodium azide |
| Purification: | Protein A/G purified from cell culture supernatant |
| Dilution Range: | IP: 1:20, WB: 1:500-1:1000 |
| Storage Instruction: | Suitable for storage at +4°C between 1-2 weeks. For longer term store at-20°C for up to 12 months. |
| Gene Symbol: | ITPK1 |
| Gene ID: | 3705 |
| Uniprot ID: | ITPK1_HUMAN |
| Post Translational Modifications | Acetylation by EP300 and CREBBP destabilizes ITPK1, and down-regulates enzymatic activity. Deacetylated by SIRT1. |
| Function | Kinase that can phosphorylate various inositol polyphosphate such as Ins(3,4,5,6)P4 or Ins(1,3,4)P3. Phosphorylates Ins(3,4,5,6)P4 at position 1 to form Ins(1,3,4,5,6)P5. This reaction is thought to have regulatory importance, since Ins(3,4,5,6)P4 is an inhibitor of plasma membrane Ca(2+)-activated Cl(-) channels, while Ins(1,3,4,5,6)P5 is not. Also phosphorylates Ins(1,3,4)P3 on O-5 and O-6 to form Ins(1,3,4,6)P4, an essential molecule in the hexakisphosphate (InsP6) pathway. Also acts as an inositol polyphosphate phosphatase that dephosphorylates Ins(1,3,4,5)P4 and Ins(1,3,4,6)P4 to Ins(1,3,4)P3, and Ins(1,3,4,5,6)P5 to Ins(3,4,5,6)P4. May also act as an isomerase that interconverts the inositol tetrakisphosphate isomers Ins(1,3,4,5)P4 and Ins(1,3,4,6)P4 in the presence of ADP and magnesium. Probably acts as the rate-limiting enzyme of the InsP6 pathway. Modifies TNF-alpha-induced apoptosis by interfering with the activation of TNFRSF1A-associated death domain. Plays an important role in MLKL-mediated necroptosis. Produces highly phosphorylated inositol phosphates such as inositolhexakisphosphate (InsP6) which bind to MLKL mediating the release of an N-terminal auto-inhibitory region leading to its activation. Essential for activated phospho-MLKL to oligomerize and localize to the cell membrane during necroptosis. |
| Protein Name | Inositol-Tetrakisphosphate 1-KinaseInositol 1 -3 -4-Trisphosphate 5/6-KinaseInositol-Triphosphate 5/6-KinaseIns(1 -3 -4p(3 5/6-Kinase |
| Database Links | Reactome: R-HSA-1855167Reactome: R-HSA-1855204Reactome: R-HSA-983231 |
| Alternative Antibody Names | Anti-Inositol-Tetrakisphosphate 1-Kinase antibodyAnti-Inositol 1 -3 -4-Trisphosphate 5/6-Kinase antibodyAnti-Inositol-Triphosphate 5/6-Kinase antibodyAnti-Ins(1 -3 -4p(3 5/6-Kinase antibodyAnti-ITPK1 antibody |
Information sourced from Uniprot.org