| Host: | Rabbit |
| Applications: | IHC/WB |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit monoclonal antibody anti-Recombinant-Histone H3.3 is suitable for use in Immunohistochemistry and Western Blot research applications. |
| Clonality : | Monoclonal |
| Clone ID : | RM2B52 |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.01M PBS, pH 7.4, 0.05% BSA, 50% Glycerol, 0.05% Sodium azide |
| Purification: | Protein A/G purified from cell culture supernatant |
| Dilution Range: | IHC: 1:50-1:100, WB: 1:500-1:1000 |
| Storage Instruction: | Suitable for storage at +4°C between 1-2 weeks. For longer term store at-20°C for up to 12 months. |
| Gene Symbol: | H3-3A.H3-3B |
| Gene ID: | 3020/3021 |
| Uniprot ID: | H33_HUMAN |
| Function | Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. |
| Protein Name | Histone H3.3 |
| Database Links | Reactome: R-HSA-1912408Reactome: R-HSA-201722Reactome: R-HSA-212300Reactome: R-HSA-2299718Reactome: R-HSA-2559580Reactome: R-HSA-2559582Reactome: R-HSA-427359Reactome: R-HSA-427389Reactome: R-HSA-427413Reactome: R-HSA-5250924Reactome: R-HSA-5334118Reactome: R-HSA-5578749Reactome: R-HSA-5617472Reactome: R-HSA-5625886Reactome: R-HSA-68616Reactome: R-HSA-73728Reactome: R-HSA-73772Reactome: R-HSA-8936459Reactome: R-HSA-8939236Reactome: R-HSA-9018519Reactome: R-HSA-912446Reactome: R-HSA-9616222Reactome: R-HSA-9670095Reactome: R-HSA-9710421Reactome: R-HSA-977225Reactome: R-HSA-9821002Reactome: R-HSA-9821993Reactome: R-HSA-983231Reactome: R-HSA-9843940Reactome: R-HSA-9843970Reactome: R-HSA-9845323 |
| Cellular Localisation | NucleusChromosome |
| Alternative Antibody Names | Anti-Histone H3.3 antibodyAnti-H3-3A antibodyAnti-H3.3A antibodyAnti-H3F3 antibodyAnti-H3F3A antibodyAnti-PP781.H3-3B antibodyAnti-H3.3B antibodyAnti-H3F3B antibody |
Information sourced from Uniprot.org