• Western blot analysis of eGFP. Sample: Protein treated by RIPA Lysis Buffer. Blocking buffer: 3% Nonfat dry milkinTBST, RT, 1h. Primary antibody: 1:1000, 4C overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG, 1:3000, RT, 1h.
  • ICC analysis of eGFPred. Sample: Transfected 293F cells, 4% PFA 20min. Antigen retrieval: Permeabilization Solution, RT, 20 min. Blocking buffer: 3% BSA in PBS, RT, 30min. Primary antibody: 1:1000, 4C overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit lgG, 1:300, RT, 1h.

Anti-Recombinant-eGFP antibody [SR8241] (STJA0018241)

SKU:
STJA0018241-100

Current Stock:
Host: Rabbit
Applications: WB/ICC/IF
Reactivity: eGFP
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Recombinant-eGFP is suitable for use in Western Blot, Immunocytochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: SR8241
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.15% ProClin300, 100 Mu g/mL BSA and 50% glycerol.
Purification: Affinity purification
Dilution Range: WB (Species independent) 1:1000-1:2000
ICC/IF (Species independent
Storage Instruction: Store at-20C for up to one year, and avoid repeated freeze-thaw cycles.
Immunogen: Recombinant protein corresponding to EGFP (enhanced green fluorescent protein)
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance