| Host: |
Rabbit |
| Applications: |
IHC/WB |
| Reactivity: |
Human/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : |
Rabbit monoclonal antibody anti-Recombinant-Nadh-Cytochrome B5 Reductase 3 is suitable for use in Immunohistochemistry and Western Blot research applications. |
| Clonality : |
Monoclonal |
| Clone ID : |
RM1H12 |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
0.01M PBS |
| Purification: |
Protein A/G purified from cell culture supernatant |
| Dilution Range: |
IHC: 1:50-1:100 |
| Storage Instruction: |
Suitable for storage at +4°C between 1-2 weeks. For longer term store at-20°C for up to 12 months. |
| Function | Catalyzes the reduction of two molecules of cytochrome b5 using NADH as the electron donor. |
| Protein Name | Nadh-Cytochrome B5 Reductase 3B5rCytochrome B5 ReductaseDiaphorase-1 |
| Database Links | Reactome: R-HSA-196836Reactome: R-HSA-211945Reactome: R-HSA-6798695 |
| Cellular Localisation | Isoform 1: Endoplasmic Reticulum MembraneLipid-AnchorCytoplasmic SideMitochondrion Outer MembraneIsoform 2: Cytoplasm |
| Alternative Antibody Names | Anti-Nadh-Cytochrome B5 Reductase 3 antibodyAnti-B5r antibodyAnti-Cytochrome B5 Reductase antibodyAnti-Diaphorase-1 antibodyAnti-CYB5R3 antibodyAnti-DIA1 antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and CYB5R3 knockdown (KD) HeLa cells using [KD Validated] CYB5R3 Rabbit polyclonal antibody (STJ11105448) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 10s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/178092/370252/STJ11105448_1__45300.1713136185.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and CYB5R3 knockdown (KD) HeLa cells using [KD Validated] CYB5R3 Rabbit polyclonal antibody (STJ11105448) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 10s.")
