Anti-Recombinant-CD8 alpha antibody [SR0555] (STJA0010555)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenKLH conjugated Synthetic peptide corresponding to Mouse CD8 alpha
General Information
| Short Description | Rabbit monoclonal anti-Recombinant-CD8 alpha for use in WB, IHC and IF in Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF |
| Host | Rabbit |
| Reactivity | Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | SR0555 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Affinity purification |
| Dilution Range | WB (M) 1:500-1:1000IHC/IF (M) 1:400-1:800 |
| Formulation | PBS with 0.15% ProClin300, 100 Mu g/mL BSA and 50% glycerol. |
| Storage Instruction | Store at-20C for up to one year, and avoid repeated freeze-thaw cycles. |
Target Information
| Gene Symbol | Cd8a |
| Gene ID | 12525 |
| Uniprot ID | CD8A_MOUSE |
| Immunogen | KLH conjugated Synthetic peptide corresponding to Mouse CD8 alpha |
Additional Info
| Function | Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide complex. The antigens presented by class I peptides are derived from cytosolic proteins while class II derived from extracellular proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of cytotoxic T-lymphocytes (CTLs). This mechanism enables CTLs to recognize and eliminate infected cells and tumor cells. In NK-cells, the presence of CD8A homodimers at the cell surface provides a survival mechanism allowing conjugation and lysis of multiple target cells. CD8A homodimer molecules also promote the survival and differentiation of activated lymphocytes into memory CD8 T-cells. |
| Protein Name | T-Cell Surface Glycoprotein Cd8 Alpha ChainT-Cell Surface Glycoprotein Lyt-2Cd Antigen Cd8a |
| Database Links | Reactome: R-MMU-198933 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinCd8a Localizes To Lipid Rafts Only When Associated With Its Partner Cd8b |
| Alternative Antibody Names | Anti-T-Cell Surface Glycoprotein Cd8 Alpha Chain antibodyAnti-T-Cell Surface Glycoprotein Lyt-2 antibodyAnti-Cd Antigen Cd8a antibodyAnti-Cd8a antibodyAnti-Lyt-2 antibodyAnti-Lyt2 antibody |
Information sourced from Uniprot.org