| Host: | CHO cells |
| Applications: | ELISA |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Cho Cells monoclonal antibody anti-Recombinant-Cardiac Troponin I is suitable for use in ELISA research applications. |
| Clonality : | Monoclonal |
| Clone ID : | 5F8 |
| Conjugation: | Unconjugated |
| Isotype: | IgG2 lambda |
| Formulation: | 50 mM Na-citrate, pH 6.0, 0.9 % NaCI, 0.095 % NaN3 (SPTN) |
| Purification: | MabSelect affinity chromatography following gel filtration |
| Storage Instruction: | Store at-20 °C to-70 °C long term. Avoid multiple freeze-thaw cycles. |
| Dissociation Constant: | 1.60 x 10-10 M (Troponin complex binding from solution) 1 x 10-12 M (Troponin complex binding from streptavidin surface) |
| Specificity: | human cardiac troponin I |
| Immunogen: | Recombinant human cardiac troponin complex protein produced by CHO-based proprietary suspension cell line. |
| Background | Troponin I is a part of the troponin complex. It binds to actin in thin myofilaments to hold the actin-tropomyosin complex in place. Because of it, myosin cannot bind actin in relaxed muscle. When calcium binds to the Troponin C it causes conformational changes which lead to dislocation of troponin I and finally tropomyosin leaves the binding site for myosin on actin leading to contraction of muscle (Wikipedia) |
Information sourced from Uniprot.org