| Post Translational Modifications | Proteolytically cleaved by caspase-3 in a cell cycle specific manner. The cleavage might be involved in the durability of the cell cycle delay. Caspase-3 cleavage is associated with abrogation of the mitotic checkpoint. The major site of cleavage is at Asp-610. Acetylation at Lys-250 regulates its degradation and timing in anaphase entry. Ubiquitinated. Degraded by the proteasome. Ubiquitinated by UBR5, promoting disassembly of the mitotic checkpoint complex from the APC/C complex. Sumoylated with SUMO2 and SUMO3. The sumoylation mediates the association with CENPE at the kinetochore. Autophosphorylated in vitro. Intramolecular autophosphorylation is stimulated by CENPE. Phosphorylated during mitosis and hyperphosphorylated in mitotically arrested cells. Phosphorylation at Ser-670 and Ser-1043 occurs at kinetochores upon mitotic entry with dephosphorylation at the onset of anaphase. |
| Function | Essential component of the mitotic checkpoint. Required for normal mitosis progression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle. One of its checkpoint functions is to inhibit the activity of the anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C, independently of its kinase activity. The other is to monitor kinetochore activities that depend on the kinetochore motor CENPE. Required for kinetochore localization of CENPE. Negatively regulates PLK1 activity in interphase cells and suppresses centrosome amplification. Also implicated in triggering apoptosis in polyploid cells that exit aberrantly from mitotic arrest. May play a role for tumor suppression. |
| Protein Name | Mitotic Checkpoint Serine/Threonine-Protein Kinase Bub1 BetaMad3/Bub1-Related Protein KinaseHbubr1Mitotic Checkpoint Kinase Mad3lProtein Ssk1 |
| Database Links | Reactome: R-HSA-141430Reactome: R-HSA-141444Reactome: R-HSA-174184Reactome: R-HSA-176409Reactome: R-HSA-179409Reactome: R-HSA-2467813Reactome: R-HSA-2500257Reactome: R-HSA-5663220Reactome: R-HSA-68877Reactome: R-HSA-9648025 |
| Cellular Localisation | CytoplasmNucleusChromosomeCentromereKinetochoreCytoskeletonMicrotubule Organizing CenterCentrosomeCytoplasmic In Interphase CellsAssociates With The Kinetochores In Early ProphaseKinetochore Localization Requires Bub1Plk1 And Knl1 |
| Alternative Antibody Names | Anti-Mitotic Checkpoint Serine/Threonine-Protein Kinase Bub1 Beta antibodyAnti-Mad3/Bub1-Related Protein Kinase antibodyAnti-Hbubr1 antibodyAnti-Mitotic Checkpoint Kinase Mad3l antibodyAnti-Protein Ssk1 antibodyAnti-BUB1B antibodyAnti-BUBR1 antibodyAnti-MAD3L antibodyAnti-SSK1 antibody |
Information sourced from Uniprot.org
