Anti-Recombinant-AGO2 antibody [RM2P22] (STJA0026283)

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STJA0026283
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Host: Rabbit
Applications: IF/IP/WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description : Rabbit monoclonal antibody anti-Recombinant-Protein Argonaute-2 is suitable for use in Immunofluorescence, Immunoprecipitation and Western Blot research applications.
Clonality : Monoclonal
Clone ID : RM2P22
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.01M PBS, pH 7.4, 0.05% BSA, 50% Glycerol, 0.05% Sodium azide
Purification: Protein A/G purified from cell culture supernatant
Dilution Range: IF: 1:50-1:200, IP: 1:20, WB: 1:500-1:1000
Storage Instruction: Suitable for storage at +4°C between 1-2 weeks. For longer term store at-20°C for up to 12 months.
Gene Symbol: AGO2
Gene ID: 27161
Uniprot ID: AGO2_HUMAN
Function Required for RNA-mediated gene silencing (RNAi) by the RNA-induced silencing complex (RISC). The 'minimal RISC' appears to include AGO2 bound to a short guide RNA such as a microRNA (miRNA) or short interfering RNA (siRNA). These guide RNAs direct RISC to complementary mRNAs that are targets for RISC-mediated gene silencing. The precise mechanism of gene silencing depends on the degree of complementarity between the miRNA or siRNA and its target. Binding of RISC to a perfectly complementary mRNA generally results in silencing due to endonucleolytic cleavage of the mRNA specifically by AGO2. Binding of RISC to a partially complementary mRNA results in silencing through inhibition of translation, and this is independent of endonuclease activity. May inhibit translation initiation by binding to the 7-methylguanosine cap, thereby preventing the recruitment of the translation initiation factor eIF4-E. May also inhibit translation initiation via interaction with EIF6, which itself binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit. The inhibition of translational initiation leads to the accumulation of the affected mRNA in cytoplasmic processing bodies (P-bodies), where mRNA degradation may subsequently occur. In some cases RISC-mediated translational repression is also observed for miRNAs that perfectly match the 3' untranslated region (3'-UTR). Can also up-regulate the translation of specific mRNAs under certain growth conditions. Binds to the AU element of the 3'-UTR of the TNF (TNF-alpha) mRNA and up-regulates translation under conditions of serum starvation. Also required for transcriptional gene silencing (TGS), in which short RNAs known as antigene RNAs or agRNAs direct the transcriptional repression of complementary promoter regions. (Microbial infection) Upon Sars-CoV-2 infection, associates with viral miRNA-like small RNA, CoV2-miR-O7a, and may repress mRNAs, such as BATF2, to evade the IFN response.
Protein Name Protein Argonaute-2
Argonaute2
Hago2
Argonaute Risc Catalytic Component 2
Eukaryotic Translation Initiation Factor 2c 2
Eif-2c 2
Eif2c 2
Paz Piwi Domain Protein
Ppd
Protein Slicer
Database Links Reactome: R-HSA-1912408
Reactome: R-HSA-203927
Reactome: R-HSA-4086398
Reactome: R-HSA-426486
Reactome: R-HSA-426496
Reactome: R-HSA-5578749
Reactome: R-HSA-5628897
Reactome: R-HSA-5687128
Reactome: R-HSA-8934593
Reactome: R-HSA-8943723
Reactome: R-HSA-8948700
Reactome: R-HSA-8986944
Reactome: R-HSA-9018519
Reactome: R-HSA-9022692
Reactome: R-HSA-9029569
Reactome: R-HSA-9725371
Reactome: R-HSA-9759811
Reactome: R-HSA-9768778
Reactome: R-HSA-9820841
Reactome: R-HSA-9824594
Reactome: R-HSA-9839394
Cellular Localisation Cytoplasm
P-Body
Nucleus
Translational Repression Of Mrnas Results In Their Recruitment To P-Bodies
Translocation To The Nucleus Requires Imp8
Alternative Antibody Names Anti-Protein Argonaute-2 antibody
Anti-Argonaute2 antibody
Anti-Hago2 antibody
Anti-Argonaute Risc Catalytic Component 2 antibody
Anti-Eukaryotic Translation Initiation Factor 2c 2 antibody
Anti-Eif-2c 2 antibody
Anti-Eif2c 2 antibody
Anti-Paz Piwi Domain Protein antibody
Anti-Ppd antibody
Anti-Protein Slicer antibody
Anti-AGO2 antibody
Anti-EIF2C2 antibody

Information sourced from Uniprot.org