Anti-RECK antibody (21-70 aa) (STJ95401)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human RECK at the amino acid range 21-70
General Information
| Short Description | Rabbit polyclonal anti-Reversion-inducing cysteine-rich protein with Kazal motifs (21-70 aa) for use in IHC, IF, WB and ELISA in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/IF/WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000IHC 1:100-1:300IF 1:200-1:1000ELISA 1:20000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | RECK |
| Gene ID | 8434 |
| Uniprot ID | RECK_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human RECK at the amino acid range 21-70 |
| Immunogen Region | 21-70 aa |
| Specificity | RECK Polyclonal Antibody detects endogenous levels of RECK protein. |
Additional Info
| Post Translational Modifications | N-glycosylated. |
| Function | Functions together with ADGRA2 to enable brain endothelial cells to selectively respond to Wnt7 signals (WNT7A or WNT7B). Plays a key role in Wnt7-specific responses: required for central nervous system (CNS) angiogenesis and blood-brain barrier regulation. Acts as a Wnt7-specific coactivator of canonical Wnt signaling by decoding Wnt ligands: acts by interacting specifically with the disordered linker region of Wnt7, thereby conferring ligand selectivity for Wnt7. ADGRA2 is then required to deliver RECK-bound Wnt7 to frizzled by assembling a higher-order RECK-ADGRA2-Fzd-LRP5-LRP6 complex. Also acts as a serine protease inhibitor: negatively regulates matrix metalloproteinase-9 (MMP9) by suppressing MMP9 secretion and by direct inhibition of its enzymatic activity. Also inhibits metalloproteinase activity of MMP2 and MMP14 (MT1-MMP). |
| Protein Name | Reversion-Inducing Cysteine-Rich Protein With Kazal MotifsHreckSuppressor Of Tumorigenicity 15 Protein |
| Database Links | Reactome: R-HSA-163125 |
| Cellular Localisation | Cell MembraneLipid-AnchorGpi-Anchor |
| Alternative Antibody Names | Anti-Reversion-Inducing Cysteine-Rich Protein With Kazal Motifs antibodyAnti-Hreck antibodyAnti-Suppressor Of Tumorigenicity 15 Protein antibodyAnti-RECK antibodyAnti-ST15 antibody |
Information sourced from Uniprot.org