| Short Description : | Rabbit polyclonal anti-Meiotic recombination protein REC8 homolog (154-203 aa) for use in WB, ELISA and IHC in Human, Rat and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/ELISA/IHC |
| Host: | Rabbit |
| Reactivity: | Human/Rat/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | 1 mg/mL |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range: | WB 1:500-2000IHC-P 1:50-300ELISA 2000-20000 |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | REC8 |
| Gene ID: | 9985 |
| Uniprot ID: | REC8_HUMAN |
| Immunogen: | The antiserum was produced against synthesized peptide derived from the human REC8 at the amino acid range 154-203 |
| Immunogen Region: | 154-203 aa |
| Specificity: | Rec8 Polyclonal Antibody detects endogenous levels of Rec8 protein. |
| Post Translational Modifications | Phosphorylated. |
| Function | Required during meiosis for separation of sister chromatids and homologous chromosomes. Proteolytic cleavage of REC8 on chromosome arms by separin during anaphase I allows for homologous chromosome separation in meiosis I and cleavage of REC8 on centromeres during anaphase II allows for sister chromatid separation in meiosis II. |
| Protein Name | Meiotic Recombination Protein Rec8 HomologCohesin Rec8p |
| Database Links | Reactome: R-HSA-1221632 |
| Cellular Localisation | NucleusChromosomeCentromereIn Meiotic ChromosomesLocalized Along Axial Elements In Prophase From The Leptotene To Diplotene StagesAt Later Prophase StagesDiakinesis And Metaphase ILocalized Along Interstitial Axes Of Chromosomes Including Both Centromere And Arm RegionsNo Longer Detected In Arm Regions In Anaphase I But Persists On Centromere Regions Until Metaphase IiLocalized To Centromeres And Spindle Poles In Endopolyploid Tumor Cells |
| Alternative Antibody Names | Anti-Meiotic Recombination Protein Rec8 Homolog antibodyAnti-Cohesin Rec8p antibodyAnti-REC8 antibodyAnti-REC8L1 antibody |
Information sourced from Uniprot.org
