• Western blot analysis of various lysates using Radixin Rabbit polyclonal antibody (STJ116977) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-RDX antibody (1-200) (STJ116977)

SKU:
STJ116977

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RDX (1-200) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RDX
Gene ID: 5962
Uniprot ID: RADI_HUMAN
Immunogen Region: 1-200
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human Radixin (NP_002897.1).
Immunogen Sequence: MPKPINVRVTTMDAELEFAI QPNTTGKQLFDQVVKTVGLR EVWFFGLQYVDSKGYSTWLK LNKKVTQQDVKKENPLQFKF RAKFFPEDVSEELIQEITQR LFFLQVKEAILNDEIYCPPE TAVLLASYAVQAKYGDYNKE IHKPGYLANDRLLPQRVLEQ HKLTKEQWEERIQNWHEEHR GMLREDSMMEYLKIAQDLEM
Post Translational Modifications Phosphorylated by tyrosine-protein kinases. Phosphorylation by ROCK2 suppresses the head-to-tail association of the N-terminal and C-terminal halves resulting in an opened conformation which is capable of actin and membrane-binding.
Function Probably plays a crucial role in the binding of the barbed end of actin filaments to the plasma membrane.
Protein Name Radixin
Database Links Reactome: R-HSA-437239
Reactome: R-HSA-9662360
Reactome: R-HSA-9662361
Cellular Localisation Cell Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Cytoplasm
Cytoskeleton
Cleavage Furrow
Cell Projection
Microvillus
Highly Concentrated In The Undercoat Of The Cell-To-Cell Adherens Junction And The Cleavage Furrow In The Interphase And Mitotic Phase
Respectively
Alternative Antibody Names Anti-Radixin antibody
Anti-RDX antibody

Information sourced from Uniprot.org

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