• Western blot analysis of various lysates using Radixin Rabbit polyclonal antibody (STJ116977) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-RDX antibody (1-200) (STJ116977)

SKU:
STJ116977

£112.50 - £524.50
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RDX
Gene ID: 5962
Uniprot ID: RADI_HUMAN
Immunogen Region: 1-200
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human Radixin (NP_002897.1).
Immunogen Sequence: MPKPINVRVTTMDAELEFAI QPNTTGKQLFDQVVKTVGLR EVWFFGLQYVDSKGYSTWLK LNKKVTQQDVKKENPLQFKF RAKFFPEDVSEELIQEITQR LFFLQVKEAILNDEIYCPPE TAVLLASYAVQAKYGDYNKE IHKPGYLANDRLLPQRVLEQ HKLTKEQWEERIQNWHEEHR GMLREDSMMEYLKIAQDLEM
Post Translational Modifications Phosphorylated by tyrosine-protein kinases. Phosphorylation by ROCK2 suppresses the head-to-tail association of the N-terminal and C-terminal halves resulting in an opened conformation which is capable of actin and membrane-binding.
Function Probably plays a crucial role in the binding of the barbed end of actin filaments to the plasma membrane.
Protein Name Radixin
Database Links Reactome: R-HSA-437239
Reactome: R-HSA-9662360
Reactome: R-HSA-9662361
Cellular Localisation Cell Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Cytoplasm
Cytoskeleton
Cleavage Furrow
Cell Projection
Microvillus
Stereocilium
Enriched At The Stereocilium Base With Very Low Levels In The Shaft Of Stereociliary Bundles
Highly Concentrated In The Undercoat Of The Cell-To-Cell Adherens Junction And The Cleavage Furrow In The Interphase And Mitotic Phase
Respectively
Alternative Antibody Names Anti-Radixin antibody
Anti-RDX antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance