Anti-RBM7 antibody (1-266) (STJ119671)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal RBM7 (1-266) antibody for use in WB and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | RBM7 |
| Gene ID | 10179 |
| Uniprot ID | RBM7_HUMAN |
| Immunogen Region | 1-266 |
| Immunogen Sequence | MGAAAAEADRTLFVGNLETK VTEELLFELFHQAGPVIKVK IPKDKDGKPKQFAFVNFKHE VSVPYAMNLLNGIKLYGRPI KIQFRSGSSHAPQDVSLSYP QHHVGNSSPTSTSPSRYERT MDNMTSSAQIIQRSFSSPEN FQRQAVMNSALRQMSYGGKF GSSPLDQSGFSPSVQSHSHS FNQSSSSQWRQGTPSSQRKV RMNSYPYLADRHYSREQRYT DHGSDHHYRGKRDDFFYED |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-266 of human RBM7 (NP_057174.1). |
Additional Info
| Tissue Specificity | Ubiquitous. |
| Post Translational Modifications | Phosphorylated at Ser-136 by MAPK14/p38-alpha-activated MAPKAPK2/MK2.this phosphorylation is stress-dependent.this phosphorylation decreases its RNA-binding capacity therefore affecting RNA nuclear exosome-mediated degradation. This phosphorylation mediates YWHAE and YWHAZ interactions. |
| Function | RNA-binding subunit of the trimeric nuclear exosome targeting (NEXT) complex, a complex that functions as an RNA exosome cofactor that directs a subset of non-coding short-lived RNAs for exosomal degradation. NEXT is involved in surveillance and turnover of aberrant transcripts and non-coding RNAs. Binds preferentially polyuridine sequences and associates with newly synthesized RNAs, including pre-mRNAs and short-lived exosome substrates such as promoter upstream transcripts (PROMPTs), enhancer RNAs (eRNAs), and 3'-extended products from small nuclear RNAs (snRNAs). Participates in several biological processes including DNA damage response (DDR) and stress response. During stress response, activation of the p38MAPK-MK2 pathway decreases RBM7-RNA-binding and subsequently the RNA exosome degradation activities, thereby modulating the turnover of non-coding transcriptome. Participates in DNA damage response (DDR), through its interaction with MEPCE and LARP7, the core subunits of 7SK snRNP complex, that release the positive transcription elongation factor b (P-TEFb) complex from the 7SK snRNP. In turn, activation of P-TEFb complex induces the transcription of P-TEFb-dependent DDR genes to promote cell viability. |
| Protein Name | Rna-Binding Protein 7Rna-Binding Motif Protein 7 |
| Database Links | Reactome: R-HSA-72163Reactome: R-HSA-9843970 |
| Cellular Localisation | NucleusNucleoplasmExcluded From The Nucleolus |
| Alternative Antibody Names | Anti-Rna-Binding Protein 7 antibodyAnti-Rna-Binding Motif Protein 7 antibodyAnti-RBM7 antibody |
Information sourced from Uniprot.org