Anti-RBM14 antibody (110-220) (STJ115125)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal RBM14 (110-220) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IF/ICC:1:100-1:500ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | RBM14 |
| Gene ID | 100526737/10432 |
| Uniprot ID | RBM14_HUMAN |
| Immunogen Region | 110-220 |
| Immunogen Sequence | VVKDYAFVHMEKEADAKAAI AQLNGKEVKGKRINVELSTK GQKKGPGLAVQSGDKTKKPG AGDTAFPGTGGFSATFDYQQ AFGNSTGGFDGQARQPTPPF FGRDRSPLRRS |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 110-220 of human RBM14 (NP_006319.1). |
Additional Info
| Tissue Specificity | Expressed in all tissues tested, including brain, heart, skeletal muscle, colon, thymus, spleen, kidney, liver, small intestine, placenta, lung and peripheral blood lymphocytes. |
| Function | Isoform 1 may function as a nuclear receptor coactivator, enhancing transcription through other coactivators such as NCOA6 and CITED1. Isoform 2, functions as a transcriptional repressor, modulating transcriptional activities of coactivators including isoform 1, NCOA6 and CITED1. Regulates centriole biogenesis by suppressing the formation of aberrant centriolar protein complexes in the cytoplasm and thus preserving mitotic spindle integrity. Prevents the formation of the STIL-CPAP complex (which can induce the formation of aberrant centriolar protein complexes) by interfering with the interaction of STIL with CPAP. Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway. Also involved in the regulation of pre-mRNA alternative splicing. |
| Protein Name | Rna-Binding Protein 14Paraspeckle Protein 2Psp2Rna-Binding Motif Protein 14Rrm-Containing Coactivator Activator/ModulatorSynaptotagmin-Interacting ProteinSyt-Interacting Protein |
| Database Links | Reactome: R-HSA-8941326 |
| Cellular Localisation | NucleusNucleolusCytoplasmIn Punctate Subnuclear Structures Often Located Adjacent To Splicing SpecklesCalled ParaspecklesCytoplasmic Localization Is Crucial For Its Function In Suppressing The Formation Of Aberrant Centriolar Protein Complexes |
| Alternative Antibody Names | Anti-Rna-Binding Protein 14 antibodyAnti-Paraspeckle Protein 2 antibodyAnti-Psp2 antibodyAnti-Rna-Binding Motif Protein 14 antibodyAnti-Rrm-Containing Coactivator Activator/Modulator antibodyAnti-Synaptotagmin-Interacting Protein antibodyAnti-Syt-Interacting Protein antibodyAnti-RBM14 antibodyAnti-SIP antibody |
Information sourced from Uniprot.org