• Western blot of rat cortical lysate showing specific immunolabeling of the ~46/48 kDa FOX3 protein.
  • Immunofluorescence of a section of rat brain stem co-labeled with Anti-FOX3 (STJA0003841, green, 1:1000) and Anti-MAP2 (STJA0003676, red, 1:5000). The Anti-FOX3 specifically labels the nuclei and the proximal cytoplasm of neuronal cells while the Anti-MAP2 labels dendrites and overlaps with FOX3 labeling the perikarya of neurons. The blue is DAPI staining of nuclear DNA.
  • Immunolabeling of cultured rat neurons showing strong nuclear and distal cytoplasmic labeling with anti-FOX3 (STJA0003841, 1:1000, red). The complete absence of astrocyte staining is shown using anti-GFAP (STJA0003659, 1:1000, green) and nuclear staining was done with DAPI (blue).
  • Cell Line: human-derived iPSC cerebral organoids. Neurofilament-H (green) , NeuN (purple) , DAPI (turquoise). Image courtesy of Caroline Krall, Johns Hopkins University
  • Cell Line: human-derived iPSC cerebral organoids. MAP2 (pink) , NeuN (yellow) , DAPI (turquoise). Image courtesy of Caroline Krall, Johns Hopkins University

Anti-RBFOX3-NeuN antibody (N-Term) [1B7] (STJA0003841)

SKU:
STJA0003841-100

£442.50
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Host: Mouse
Applications: WB/IHC/ICC
Reactivity: Bovine/Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-FOX3-NeuN (N-Term) is suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry research applications.
Clonality: Monoclonal
Clone ID: 1B7
Conjugation: Unconjugated
Isotype: IgG2b
Formulation: 100 ul in PBS + 50% Glycerol and 5 mm Sodium Azide
Purification: This antibody was protein g purified culture from supernatant.
Dilution Range: WB 1:2000
IHC 1:200-1:1000
ICC 1:200-1:1000
IP
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RBFOX3
Gene ID: 146713
Uniprot ID: RFOX3_HUMAN
Immunogen Region: N-Term
Immunogen: Recombinant protein taken from the N-terminus of human FOX3 expressed in and purified from E. Coli
Function Pre-mRNA alternative splicing regulator. Regulates alternative splicing of RBFOX2 to enhance the production of mRNA species that are targeted for nonsense-mediated decay (NMD).
Protein Name Rna Binding Protein Fox-1 Homolog 3
Fox-1 Homolog C
Neuronal Nuclei Antigen
Neun Antigen
Database Links Reactome: R-HSA-9768919
Cellular Localisation Nucleus
Cytoplasm
Largely Restricted To Neuronal Nuclei
However
Significant Cytoplasmic Localization In Neurons From Brains From Hiv-Infected Individuals With Cognitive Impairment
Alternative Antibody Names Anti-Rna Binding Protein Fox-1 Homolog 3 antibody
Anti-Fox-1 Homolog C antibody
Anti-Neuronal Nuclei Antigen antibody
Anti-Neun Antigen antibody
Anti-RBFOX3 antibody

Information sourced from Uniprot.org

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