• Western blot analysis of various lysates, using RARS rabbit polyclonal antibody (STJ28229) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using RARS antibody (STJ28229) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human lung cancer using RARS antibody (STJ28229) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse brain using RARS antibody (STJ28229) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of HeLa cells using RARS rabbit polyclonal antibody (STJ28229) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using RARS rabbit polyclonal antibody (STJ28229) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-RARS antibody (1-300) (STJ28229)

SKU:
STJ28229

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IHC-P/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IHC-P:1:50-1:200
IF/ICC:1:10-1:100
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RARS1
Gene ID: 5917
Uniprot ID: SYRC_HUMAN
Immunogen Region: 1-300
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human RARS (NP_002878.2).
Immunogen Sequence: MDVLVSECSARLLQQEEEIK SLTAEIDRLKNCGCLGASPN LEQLQEENLKLKYRLNILRK SLQAERNKPTKNMINIISRL QEVFGHAIKAAYPDLENPPL LVTPSQQAKFGDYQCNSAMG ISQMLKTKEQKVNPREIAEN ITKHLPDNECIEKVEIAGPG FINVHLRKDFVSEQLTSLLV NGVQLPALGENKKVIVDFSS PNIAKEMHVGHLRSTIIGES ISRLFEFAGYDVLRLNHVG
Function Forms part of a macromolecular complex that catalyzes the attachment of specific amino acids to cognate tRNAs during protein synthesis. Modulates the secretion of AIMP1 and may be involved in generation of the inflammatory cytokine EMAP2 from AIMP1.
Protein Name Arginine--Trna Ligase - Cytoplasmic
Arginyl-Trna Synthetase
Argrs
Database Links Reactome: R-HSA-2408522
Reactome: R-HSA-379716
Reactome: R-HSA-9856649
Cellular Localisation Cytoplasm
Cytosol
Alternative Antibody Names Anti-Arginine--Trna Ligase - Cytoplasmic antibody
Anti-Arginyl-Trna Synthetase antibody
Anti-Argrs antibody
Anti-RARS1 antibody
Anti-RARS antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance