| Host: | Rabbit |
| Applications: | WB/IHC-P/ELISA |
| Reactivity: | Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000 IHC-P:1:50-1:200 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | RANBP2 |
| Gene ID: | 5903 |
| Uniprot ID: | RBP2_HUMAN |
| Immunogen Region: | 2900-3050 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 2900-3050 of human RANBP2 (NP_006258.3). |
| Immunogen Sequence: | SDEEVVHNEDIHFEPIVSLP EVEVKSGEEDEEILFKERAK LYRWDRDVSQWKERGVGDIK ILWHTMKNYYRILMRRDQVF KVCANHVITKTMELKPLNVS NNALVWTASDYADGEAKVEQ LAVRFKTKEVADCFKKTFEE CQQNLMKLQKG |
| Post Translational Modifications | Polyubiquitinated by PRKN, which leads to proteasomal degradation. The inner channel of the NPC has a different redox environment from the cytoplasm and allows the formation of interchain disulfide bonds between some nucleoporins, the significant increase of these linkages upon oxidative stress reduces the permeability of the NPC. |
| Function | E3 SUMO-protein ligase which facilitates SUMO1 and SUMO2 conjugation by UBE2I. Involved in transport factor (Ran-GTP, karyopherin)-mediated protein import via the F-G repeat-containing domain which acts as a docking site for substrates. Binds single-stranded RNA (in vitro). May bind DNA. Component of the nuclear export pathway. Specific docking site for the nuclear export factor exportin-1. Inhibits EIF4E-dependent mRNA export. Sumoylates PML at 'Lys-490' which is essential for the proper assembly of PML-NB. Recruits BICD2 to the nuclear envelope and cytoplasmic stacks of nuclear pore complex known as annulate lamellae during G2 phase of cell cycle. Probable inactive PPIase with no peptidyl-prolyl cis-trans isomerase activity. |
| Protein Name | E3 Sumo-Protein Ligase Ranbp2 358 Kda Nucleoporin Nuclear Pore Complex Protein Nup358 Nucleoporin Nup358 Ran-Binding Protein 2 Ranbp2 P270 |
| Database Links | Reactome: R-HSA-1169408 Reactome: R-HSA-141444 Reactome: R-HSA-159227 Reactome: R-HSA-159230 Reactome: R-HSA-159231 Reactome: R-HSA-159236 Reactome: R-HSA-165054 Reactome: R-HSA-168271 Reactome: R-HSA-168276 Reactome: R-HSA-168325 Reactome: R-HSA-168333 Reactome: R-HSA-170822 Reactome: R-HSA-180746 Reactome: R-HSA-180910 Reactome: R-HSA-191859 Reactome: R-HSA-2467813 Reactome: R-HSA-2500257 Reactome: R-HSA-3108214 Reactome: R-HSA-3232142 Reactome: R-HSA-3301854 Reactome: R-HSA-3371453 Reactome: R-HSA-4085377 Reactome: R-HSA-4551638 Reactome: R-HSA-4570464 Reactome: R-HSA-4615885 Reactome: R-HSA-5578749 Reactome: R-HSA-5619107 Reactome: R-HSA-5663220 Reactome: R-HSA-6784531 Reactome: R-HSA-68877 Reactome: R-HSA-9609690 Reactome: R-HSA-9610379 Reactome: R-HSA-9648025 Reactome: R-HSA-9705671 Reactome: R-HSA-9725370 |
| Cellular Localisation | Nucleus Nucleus Membrane Nuclear Pore Complex Nucleus Envelope Detected In Diffuse And Discrete Intranuclear Foci Cytoplasmic Filaments |
| Alternative Antibody Names | Anti-E3 Sumo-Protein Ligase Ranbp2 antibody Anti-358 Kda Nucleoporin antibody Anti-Nuclear Pore Complex Protein Nup358 antibody Anti-Nucleoporin Nup358 antibody Anti-Ran-Binding Protein 2 antibody Anti-Ranbp2 antibody Anti-P270 antibody Anti-RANBP2 antibody Anti-NUP358 antibody |
Information sourced from Uniprot.org