| Host: | Rabbit |
| Applications: | WB/IHC-P/ELISA |
| Reactivity: | Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000IHC-P:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | RANBP2 |
| Gene ID: | 5903 |
| Uniprot ID: | RBP2_HUMAN |
| Immunogen Region: | 2900-3050 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 2900-3050 of human RANBP2 (NP_006258.3). |
| Immunogen Sequence: | SDEEVVHNEDIHFEPIVSLP EVEVKSGEEDEEILFKERAK LYRWDRDVSQWKERGVGDIK ILWHTMKNYYRILMRRDQVF KVCANHVITKTMELKPLNVS NNALVWTASDYADGEAKVEQ LAVRFKTKEVADCFKKTFEE CQQNLMKLQKG |
| Post Translational Modifications | Polyubiquitinated by PRKN, which leads to proteasomal degradation. The inner channel of the NPC has a different redox environment from the cytoplasm and allows the formation of interchain disulfide bonds between some nucleoporins, the significant increase of these linkages upon oxidative stress reduces the permeability of the NPC. |
| Function | E3 SUMO-protein ligase which facilitates SUMO1 and SUMO2 conjugation by UBE2I. Involved in transport factor (Ran-GTP, karyopherin)-mediated protein import via the F-G repeat-containing domain which acts as a docking site for substrates. Binds single-stranded RNA (in vitro). May bind DNA. Component of the nuclear export pathway. Specific docking site for the nuclear export factor exportin-1. Inhibits EIF4E-dependent mRNA export. Sumoylates PML at 'Lys-490' which is essential for the proper assembly of PML-NB. Recruits BICD2 to the nuclear envelope and cytoplasmic stacks of nuclear pore complex known as annulate lamellae during G2 phase of cell cycle. Probable inactive PPIase with no peptidyl-prolyl cis-trans isomerase activity. |
| Protein Name | E3 Sumo-Protein Ligase Ranbp2358 Kda NucleoporinNuclear Pore Complex Protein Nup358Nucleoporin Nup358Ran-Binding Protein 2Ranbp2P270 |
| Database Links | Reactome: R-HSA-1169408Reactome: R-HSA-141444Reactome: R-HSA-159227Reactome: R-HSA-159230Reactome: R-HSA-159231Reactome: R-HSA-159236Reactome: R-HSA-165054Reactome: R-HSA-168271Reactome: R-HSA-168276Reactome: R-HSA-168325Reactome: R-HSA-168333Reactome: R-HSA-170822Reactome: R-HSA-180746Reactome: R-HSA-180910Reactome: R-HSA-191859Reactome: R-HSA-2467813Reactome: R-HSA-2500257Reactome: R-HSA-3108214Reactome: R-HSA-3232142Reactome: R-HSA-3301854Reactome: R-HSA-3371453Reactome: R-HSA-4085377Reactome: R-HSA-4551638Reactome: R-HSA-4570464Reactome: R-HSA-4615885Reactome: R-HSA-5578749Reactome: R-HSA-5619107Reactome: R-HSA-5663220Reactome: R-HSA-6784531Reactome: R-HSA-68877Reactome: R-HSA-9609690Reactome: R-HSA-9610379Reactome: R-HSA-9648025Reactome: R-HSA-9705671Reactome: R-HSA-9725370 |
| Cellular Localisation | NucleusNucleus MembraneNuclear Pore ComplexNucleus EnvelopeDetected In Diffuse And Discrete Intranuclear FociCytoplasmic Filaments |
| Alternative Antibody Names | Anti-E3 Sumo-Protein Ligase Ranbp2 antibodyAnti-358 Kda Nucleoporin antibodyAnti-Nuclear Pore Complex Protein Nup358 antibodyAnti-Nucleoporin Nup358 antibodyAnti-Ran-Binding Protein 2 antibodyAnti-Ranbp2 antibodyAnti-P270 antibodyAnti-RANBP2 antibodyAnti-NUP358 antibody |
Information sourced from Uniprot.org