| Host: |
Rabbit |
| Applications: |
IHC/WB |
| Reactivity: |
Human/Rat/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-RalA is suitable for use in Immunohistochemistry and Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Lyophilised |
| Purification: |
IgG purified |
| Dilution Range: |
IHC, WB. A dilution of 1:4000 is recommended for WB and i:2000 for IHC-P. The optimal dilution should be determined by the end user. Not yet tested in other applications. |
| Storage Instruction: |
Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
| Gene Symbol: |
RALA |
| Gene ID: |
5898 |
| Uniprot ID: |
RALA_HUMAN |
| Specificity: |
Specific for Ral-A. |
| Immunogen: |
A synthetic peptide from human RalA conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat and mouse. |
| Function | Multifunctional GTPase involved in a variety of cellular processes including gene expression, cell migration, cell proliferation, oncogenic transformation and membrane trafficking. Accomplishes its multiple functions by interacting with distinct downstream effectors. Acts as a GTP sensor for GTP-dependent exocytosis of dense core vesicles. The RALA-exocyst complex regulates integrin-dependent membrane raft exocytosis and growth signaling. Key regulator of LPAR1 signaling and competes with GRK2 for binding to LPAR1 thus affecting the signaling properties of the receptor. Required for anchorage-independent proliferation of transformed cells. During mitosis, supports the stabilization and elongation of the intracellular bridge between dividing cells. Cooperates with EXOC2 to recruit other components of the exocyst to the early midbody. During mitosis, also controls mitochondrial fission by recruiting to the mitochondrion RALBP1, which mediates the phosphorylation and activation of DNM1L by the mitotic kinase cyclin B-CDK1. |
| Protein Name | Ras-Related Protein Ral-A |
| Database Links | Reactome: R-HSA-1445148Reactome: R-HSA-171007Reactome: R-HSA-8950505 |
| Cellular Localisation | Cell MembraneLipid-AnchorCytoplasmic SideCleavage FurrowMidbodyMidbody RingMitochondrionPredominantly At The Cell Surface In The Absence Of LpaIn The Presence Of LpaColocalizes With Lpar1 And Lpar2 In Endocytic VesiclesMay Colocalize With Cntrl/Centriolin At The Midbody RingHoweverLocalization At The Midbody At Late Cytokinesis Was Not ConfirmedRelocalizes To The Mitochondrion During Mitosis Where It Regulates Mitochondrial Fission |
| Alternative Antibody Names | Anti-Ras-Related Protein Ral-A antibodyAnti-RALA antibodyAnti-RAL antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
